An ion optical arrangement (1) for use in a mass spectrometer comprises electrodes (11, 12, 14) comprising a multipole arrangement defining an ion optical axis, and a voltage source for providing voltages to the electrodes to produce electric fields. The ion optical arrangement is configured for producing a radio frequency electric focusing field for focusing ions on the ion optical axis. The radio frequency electric focusing field has a varying frequency so as to reduce any mass dependence of ion trajectories through the ion optical arrangement. The ion optical arrangement may further be configured for producing a static electric field in response to a DC bias voltage applied to the multipole arrangement. A superimposed varying electric field may be produced by superimposing an AC voltage upon the DC bias voltage.

A mass spectrometry device includes a vacuum container in which an ionizer, a mass separator and a detector are arranged, and includes an opening-closing portion that is provided at the vacuum container and switches between a locked state of being locked in a close state and an unlocked state by a difference in pressure between inside and outside of the vacuum container, and a cooling gas introducer that introduces a cooling gas for cooling the ionizer into the vacuum container such that the opening-closing portion is kept in the locked state.

A sampling period of an A/D converter is set in accordance with an ion pulse ejection operation of a collision cell of an accumulation type. Start timing of the sampling period is changed in accordance with a selected m/z of a second mass analysis unit. In addition, end timing of the sampling period may be changed in accordance with the selected m/z of the second mass analysis unit. In place of the sampling period, a data cut-out period may be changed.

In a mass spectrometer provided with a measurement section (1) including a collision cell (17) and a mass separator (20-23) for a mass spectrometric analysis of product ions, a CES-method-condition determiner (321) determines collision-energy (CE) values for a collision energy spread (CES) method according to given conditions including the range and number of CE values, in such a manner that n+1 CE values to be used when the number is n+1 (where n is an integer equal to or greater than three) include n collision-energy values used when the number is n and one additional CE value different from the n CE values. An analysis controller (30) sequentially changes the collision energy to the n+1 CE values and controls the measurement section to execute an MS/MS analysis under each CE value. A data processor (33) obtains a cumulative mass spectrum by accumulating mass spectra respectively obtained under different CE values.

A method of mass spectrometry is disclosed comprising: performing a plurality of cycles of operation during a single experimental run, wherein each cycle comprises: mass selectively transmitting precursor ions of a single mass, or range of masses, through or out of a mass separator or mass filter at any given time, wherein the mass separator or mass filter is operated such that the single mass or range of masses transmitted therefrom is varied with time; and mass analysing ions.

An elemental mass spectrometer uses a mass filter to select ions from ions received from an ion source and transmit the selected ions. A reaction or collision cell receives the transmitted ions and reacts or collides these with a gas to provide product ions thereby. A mass analyzer receives the product ions, analyzes them and provides at least one output based on detection of the analyzed ions. The elemental mass spectrometer is operated to provide a first output from the mass analyzer measuring ions within a first analysis range of mass-to-charge to provide a second output from the mass analyzer measuring ions within a second analysis range of mass-to-charge ratios and to correct the first output for interference on the basis of the second output.

An analysis method includes: performing liquid chromatography using a mobile phase including an adsorption prevention agent for preventing adsorption of a sample including a compound having a phosphate group to metal; and performing mass spectrometry on an eluate of the liquid chromatography. The adsorption prevention agent includes an oxalic acid or a salt of the oxalic acid.

An ion filter for a mass spectrometer, the apparatus comprising an ion modifier; an ion selector configured to select a subset of a sample of ions based on their mobility in a gas; and a controller configured to operate the ion modifier in a first mode to modify the ions selected by the ion selector to provide daughter ions, and configured to operate the ion modifier in a second mode to output the ions selected by the ion selector; wherein the ion filter is adapted for providing output ions from the ion modifier to an intake of a mass spectrometer.

An ion spectrometer includes: a reaction chamber to dissociate ions derived from a sample component by causing the ions to react with radical species; a cylindrical part constituting a part of the reaction chamber and having openings at both ends; a plurality of electrodes disposed inside the cylindrical part to surround an axis that is linear along an extending direction of the cylindrical part, the electrodes extending in a direction along the axis; a heating part to heat the plurality of electrodes; a pair of electrode holding parts respectively provided in openings at both ends of the cylindrical part and having holes into which electrode support pins described later are respectively inserted; and the electrode support pins of a rod shape each provided on a surface facing the pair of electrode holding parts in each of the plurality of electrodes and extending parallel to the axis.

Methods are described for measuring the amount of C peptide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying C peptide in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques.