The present invention refers to field of assisted reproductive technologies, in particular to a sperm storage media, and describes mTOR enhancers, preferably the mTOR activator MHY 1485, as supplement for storage media, said supplemented media increasing sperm motility and viability during short-term room temperature storage, while sustaining the metabolic rates, without changing its fertilization potential (capacitation) and DNA integrity.

Provided are a cryopreservation liquid for bovine reproductive cells such as bovine sperms and a cryopreservation method thereof. Adopted is a cryopreservation liquid comprising: 0.3 to 0.9 w/w % of an amphoteric polyelectrolyte (an antifreeze polyamino acid), which is ε-poly-L-lysine having a number average molecular weight of 1,000 to 20,000 wherein 50 to 99 mol % of amino groups of the ε-poly-L-lysine are blocked as carboxylated by having been reacted with the succinic anhydride; and 2 to 4 w/w % of glycerol, as dissolved in a physiological solution. A preferred embodiment of the cryopreservation method comprises steps of: primary diluting, in which bovine semen is diluted to 2.5 to 10 times with a physiological solution and kept at 2 to 8° C.; and secondary diluting, in which the bovine semen is diluted to 5 to 20 times while being kept at 2 to 8° C., by adding dropwise a physiological solution containing the amphoteric polyelectrolyte (antifreeze polyamino acid) and glycerol to a suspension obtained in the primary diluting.

The present disclosure provides a method for producing an electrolyte solution which can contain a high concentration of microbubbles, and a method for producing a microbubble-containing solvent which can be used for preparing the electrolyte solution, by suppressing decrease in microbubbles during filtration.

A composition for restoring or increasing tissue perfusion is provided. The composition includes polyethylene glycol polymers (PEG) with a molecular weight of 18,000-100,000 Da at a concentration of 5-20% by weight; PEG with a molecular weight of 1,000-10,000 Da at a concentration of 1-30% by weight; and water, wherein said PEG with a molecular weight of 18,000-100,000 Da and said PEG with a molecular weight of 1,000-10,000 Da are dissolved or dispersed in said water.

Provided herein are compositions comprising ergothioneine (e.g., a natural form of purified L-ergothioneine) and methods of using such compositions, e.g., for improving cell viability, maintaining telomere length, or treating telomere-related disorders. In some embodiments, the ergothioneine has a purity of at least or about 98%.

Electrochemical apparatus and method provide a source of electrons to a living organ in the process of transplantation. The organ, depleted of antioxidants, is placed into a vessel supplied with appropriate physiological solution and apparatus that create antioxidative environment.

Compositions, containers and kits for low temperature storage of a specified quantity of packed red blood cells (RBCs) include L-camitine, HES and blood plasma proteins. Methods of storing a packed RBC blood product, transfusing a packed RBC blood product into a subject, and treating anemia, ischemia, hypoxia, a hemoglobin disorder, or a hematopoietic disorder in a subject (e.g., a human) include the compositions, containers, kits and blood products.

The present invention relates to a solution for the preservation of cells from placental blood, from bone marrow and from peripheral blood.

The present invention provides a method and apparatus for tissue, such as an allograft, storage and preservation for extended periods of time at room temperature in a sterile tissue culture chamber. The invention further provides a process for maintaining the sterility of tissue using the apparatus as described.

The present invention provides a prophylactic or therapeutic agent for hypoxic injury, ischemia-reperfusion injury or inflammation, an agent for protecting cells for transplantation, and an agent for preserving organism. A prophylactic or therapeutic agent for hypoxic injury, ischemia-reperfusion injury or inflammation, an agent for protecting cells for transplantation, or an agent for preserving organism, containing, as an active ingredient, at least one kind selected from a heterocyclic compound represented by the formula (I) ##STR00001##
wherein each symbol is as described in the DESCRIPTION, or a salt thereof; an isothiocyanate compound represented by the formula S═C═N—R.sup.5 (II) wherein the symbol is as described in the DESCRIPTION; and a TRPA1 agonist.