Method and device and fluid for recovering a harvested kidney, for example from a cardiac arrest carcass, wherein the kidney has been exposed to warm ischemia during 4 hours or more. At the backtable after harvesting, there is injected lys-plasminogen to the kidney and after 15 minutes, t-PA is injected. A hyperoncotic circulation fluid comprising albumin and electrolytes is added and circulated through the kidney together with said lys-plasminogen and t-PA, whereby the circulation pressure is increased from 20 mmHg to 70 mmHg during 30 to 75 minutes, for example in steps of 5 mmHg per 5 minutes. Then, the kidney is evaluated by conventional criteria.

A method for recovering an organ harvested from a donor, wherein the organ has been retrieved at least two hours after the donor had circulation arrest, comprising the steps of providing lys-plasminogen to the organ in a first hyperoncotic fluid, followed by tPA in a second hyperoncotic fluid. A third hyperoncotic fluid comprising albumin and electrolytes is circulated through the organ in a first restoration step, and in a second restoration step, a fourth hyperoncotic fluid comprising oxygenated red blood cells is circulated through the organ. Then, the organ is evaluated by conventional criteria. A device and a fluid for use in the method is also disclosed.

A frame includes a ring-shaped body part locatable to surround the periphery of an organ, and at least one tube clamp part mounted on the body part. Each tube clamp part includes a tube holder that holds a tube. The tube clamp part is mounted so as to be movable in the circumferential direction of the body part. Thus, a tube connected to the organ can be fixed at an appropriate position to the tube clamp part. This stabilizes the relative positions of the organ and the tube. Accordingly, it is possible to reduce the probability of the organ becoming damaged due to a strain on the organ.

A test bench assembly for simulating cardiac surgery includes a passive heart having at least one pair of cardiac chambers with an atrial chamber and a ventricular chamber. A reservoir is adapted to house working fluid. A pressure generator fluidically connects both to the ventricular chamber of the passive heart and to the reservoir. A pressure regulation device provides working fluid in input to the atrial chamber with preload pressure, and working fluid in output from the ventricular chamber with afterload pressure. The pressure regulation device fluidically connects both to the atrial chamber of the passive heart and to the ventricular chamber of the passive heart. The pressure regulation device has a single compliant element for each pair of cardiac chambers, which provides working fluid with both preload, and afterload pressures.

An apparatus (1) for ex-vivo measurement of performance of a donor heart (2) has a heart holder (6) in a receptacle (7) for holding a human donor heart and a bag (17) for placement into a left ventricle of the heart. The bag has a bag interior space communicating with an interior space of a fluid tight, compressible and expandable container (19. The apparatus further includes a sensor (21) for measuring compression and expansion of the container. Using the apparatus cardiac outwit can be measured by measuring expansion and contraction of the container. A method for ex-vivo measurement of performance of a donor heart is also described. The method may include controlling the preload and/or the afterload.

Disclosed are an in-vitro cardiopulmonary combined perfusion system and perfusion method. The in-vitro cardiopulmonary combined perfusion system includes an organ cabin, a circulation cabin, a control cabin, a simple breathing cabin, a display and control panel, and a base. The organ cabin is connected with the circulation cabin, the control cabin and the simple breathing cabin. The control cabin is connected with the display and control panel. The organ cabin, the circulation cabin, the control cabin, the simple breathing cabin, and the display and control panel are mounted on the base.

A dynamic temperature regulating device is for use in association with a temperature-controlled container. The dynamic temperature regulating device includes at least one heat source, at least one heat sink, a heat transfer medium and a control system. At least one of the heat source and the heat sink is a PCM (phase change material). The heat transfer medium is in thermal communication with and operably connected to the at least one heat source and the at least one heat sink. The control system is for controlling the selective thermal communication with the at least one heat source and with the at least one heat sink to regulate the temperature of the temperature-controlled container. A detachable PCM contained volumes includes a sealed housing, a phase change material and a heat transfer medium and functions as a PCM thermal energy storage volume.

A test bench assembly for simulating cardiac surgery includes a passive heart having at least one pair of cardiac chambers with an atrial chamber and a ventricular chamber. A reservoir is adapted to house working fluid. A pressure generator fluidically connects both to the ventricular chamber of the passive heart and to the reservoir. A pressure regulation device provides working fluid in input to the atrial chamber with preload pressure, and working fluid in output from the ventricular chamber with afterload pressure. The pressure regulation device fluidically connects both to the atrial chamber of the passive heart and to the ventricular chamber of the passive heart. The pressure regulation device has a single compliant element for each pair of cardiac chambers, which provides working fluid with both preload, and afterload pressures.

An organ container includes a bottomed tubular outer container and a bottomed tubular inner container that is fitted into the outer container. A fluid flow path is formed between an outer peripheral surface of the inner container and an inner peripheral surface of the outer container. The outer container has an inlet that communicates between the fluid flow path and the outside, and an outlet that communicates between the fluid flow path and the outside.

The present invention relates to a method for cryopreserving and thawing an organ-on-a-chip that has a three-dimensional tissue structure and function. Specifically, the present invention relates to a method for cryopreserving and thawing an organ-on-a-chip that comprises cells and hydrogel and has a microchannel structure, whereby the organ-on-a-chip has an excellent effect of being able to maintain the structure and function of the three-dimensional tissue before and after cryopreservation and thawing.