A liquid-tight case includes a housing to enclose a tissue device, which has a first channel network and a second channel network in fluidic communication with the first channel network. The liquid-tight case also includes a first inlet port, a second inlet port, and at least one outlet port. The first inlet port is formed at or coupled with the housing, and configured to be in fluidic communication with the first channel network of the tissue device. The second inlet port is formed at or coupled with the housing, and configured to be in fluidic communication with the second channel network of the tissue device. The at least one outlet port is formed at or coupled with the housing. The liquid-tight case allows the tissue device to have more flexible and more complex design with more suitable or modified materials for cell migration and tissue formation.

Disclosed herein are devices, apparatuses, and/or systems for delivering aerosolized fluorocarbons (FCs). The systems can include a container, an aerosolizer, and an introduction assembly. The aerosolizer can be fluidically coupled to the container and configured to aerosolize fluids from the container. The introduction assembly can be fluidically coupled to the aerosolizer and configured to introduce aerosolized fluids into the pulmonary tissue of a subject. Methods include aerosolizing a FC using an aerosolizer, delivering the FC to the pulmonary tissue of the subject, and contacting the pulmonary tissue of the subject with the FC. Some embodiments include cooling the pulmonary tissue. Cooling the pulmonary tissue can provide a therapeutic benefit to a living subject or it can preserve the pulmonary tissue of a deceased subject for transplantation.

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

A temperature sensor for monitoring an organ or tissue is configured to measure a temperature inside of a container configured to contain the organ or tissue. The temperature sensor is disposed exterior to the organ container and the temperature sensor is a non-contact temperature sensor. The temperature sensor may be part of an apparatus for perfusing, transporting, and/or storing an organ or tissue. A coolant container may have an aperture through which the temperature sensor measures a temperature of at least one of the organ or tissue or a perfusate fluid surrounding the organ or tissue. The temperature sensor is preferably an infrared temperature sensor. Multiple temperature sensors may be provided that measure the temperature organ or tissue or perfusate fluid surrounding the organ or tissue, for example in case one of the temperature sensors fails.

A conditioning device for conditioning an exposed stretch of a blood vessel has an envelope and at least one conveying channel extending entirely within the envelope. The envelope has a first layer having a first surface facing the exposed stretch, and a second layer having a second surface, opposite to the first surface. The at least one conveying channel has at least one first aperture leading out of the envelope, so that the at least one conveying channel is suppliable with working fluid through the at least one first aperture. The first layer is permeable to at least one component of the working fluid, so that, when the working fluid is in the at least one conveying channel, the at least one component, permeating the first layer, diffuses until reaching the first surface to apply a conditioning action on the exposed stretch of the blood vessel.

An organ container includes flexible films and a tube holder. The films hold an organ in contact with the surface of the organ. The tube holder holds tubes that extend between the organ in the films and an outside of the films. This makes it possible to hold and preserve the organ while allowing a liquid to be perfused through the organ via the tubes. It is also possible to reduce movements of the organ relative to the organ container and to reduce damage to the organ.

A device for support of an organ ex vivo comprises a chamber with a first engagement feature. A support structure includes a second engagement feature. The first and second engagement features may be engaged so that the chamber body is carried by the support structure for rotation. The chamber body comprises first and second chamber components. In a first orientation of the chamber body, the first chamber component provides support for a first surface of the organ, and the second chamber component is removable so that a second surface of the organ is exposed to manipulation from outside the chamber body. In a second orientation of the chamber body, the second chamber component provides support for the second surface of the organ, and the first chamber component is removable so that the first surface of the organ is exposed to manipulation from outside the chamber body.

The present disclosure relates to devices and methods for increasing the probability of a successful organ donation by decreasing organs' warm ischemia time. Furthermore, the present disclosure relates to increasing the pool of eligible donors, including donors who had prior chest surgeries or prior abdominal surgeries. The present disclosure provides a catheter extending through a cannula, wherein the cannula delivers cold perfusion fluid and the catheter blocks the aorta, forcing the cold perfusion fluid to pass to organs in the donor's abdomen. The present disclosure further provides for advancing the catheter from a retracted position to an extended position, and creating a watertight seal at the cannula to prevent backflow of cold perfusion fluid.

Described are systems and methods for monitoring administration of nitric oxide (NO) to ex vivo fluids. Examples of such fluids include blood in extracorporeal membrane oxygenation (ECMO) circuits or perfusion fluids used for preserving ex vivo organs prior to transplanting in a recipient. The systems and methods described herein provide for administering nitric oxide to the fluid, monitoring nitric oxide or a nitric oxide marker in the fluid, and adjusting the nitric oxide administration.

Described are methods of performing perfusion on a heart having a left atrium, a right atrium, a pulmonary artery. The methods are performed on a device configured for selectively performing perfusion in at least a Langendorff mode and a right-sided working mode. The methods include performing profusion on a heart in a right-side working mode of the device in which an aortic line is open, a left atrial line is closed, and a reservoir return line is closed.