A bioreactor device includes a solid substrate having a first face and a second face. The solid substrate at least partially defines a perfusion channel, a plurality of chambers, a fluidic inlet, and a fluidic outlet. A first sheet disposed over the first face and a second sheet disposed over the second face. Characteristically, the combination of the solid substrate, the first sheet and the second sheet define the perfusion channel and each chamber of the plurality of chambers. The plurality of chambers are arranged in rows of chambers in which adjacent chambers are positioned at opposite side of the perfusion channel. The perfusion channel extends from the fluidic inlet and the fluidic outlet having a serpentine path along each row of chambers with each chamber being in fluid communication with the perfusion channel.

A connector (1) includes: a tube (10) configured to be arranged in an interior of a vascular channel; a tubular body (30) having an inner wall surface configured to, together with an outer wall surface of the tube (10), sandwich the vascular channel when the tube (10) is arranged in the interior of the vascular channel; and a balloon (20), configured to be arranged on the outer wall surface of the tube (10) or the inner wall surface of the tubular body (30), and radially expand for performing sealing (i) between the soft tubular member and the inner wall surface of the tubular body (30) and (ii) between the soft tubular member and the outer wall surface of the tube (10). A fluid supply system (100) may include the connector (1), and a perfusion solution supply device (120) connected to the connector (1) and configured to supply a perfusion solution into the interior of the vascular channel.

A perfusion apparatus includes a reservoir and a perfusion-liquid feeding path that feeds a perfusion liquid from the reservoir to an organ. The perfusion-liquid feeding path includes a feeding pipe and a deaeration unit that is interposed in the feeding pipe and includes an air chamber and a deaeration pump. The deaeration pump is a roller tube pump in which a plurality of rollers squeeze a flexible gas pipe while rotating, to cause a flow of gas in the gas pipe, and discharges gas from a gas layer in an upper portion of the air chamber to outside. This makes it possible to discharge gas in the air chamber while keeping the perfusion-liquid feeding path hermetic. Therefore, it is possible to remove bubbles in the perfusion liquid fed to the organ without limiting the height of the organ and without stopping feeding the perfusion liquid to the organ.

A gas exchanger for a perfusion circuit for circulating organ preservation solution to and from a harvested donor organ, the gas exchanger comprising: (i) an inlet for receiving oxygen and CO from an oxygen reservoir and a carbon monoxide (CO) reservoir, (ii) an inlet for receiving the organ preservation solution from the perfusion circuit, (iii) an outlet gas out to environment, and (iv) an outlet for delivering the organ preservation solution treated with oxygen and CO (treated preservation solution) to the perfusion circuit. Also, a system comprising the gas exchanger and the perfusion circuit for circulating the organ preservation solution to and from the harvested donor organ and a method to preserve a donor organ comprising perfusing the donor organ with a mixture of carbon monoxide and oxygen at room temperature.

The present invention discloses an afterload device for ex situ heart perfusion, which includes an afterload energy storage module, a pressure regulation module and two flow rate regulators that are connected through a conduit. The afterload energy storage module includes a rigid closed container and a flexible container. The perfusion fluid flows into the flexible container from one side thereof through the conduit, and the perfusion fluid flows out from the flexible container at another side thereof as the flexible container is subjected to the action of the medium pressure in the rigid hermetic container. The pressure regulation module includes two pressure regulation valves and a compressible-medium source, for regulating the pressure range within the rigid hermetic container to be always maintained between the set diastolic pressure and the set systolic pressure.

An embalming machine including a housing, at least one fluid tank supported by the housing, a plurality of hose segments disposed at least partially within the housing, a centrifugal fluid transfer pump, a pressure gauge, and a first solenoid is disclosed. The pump is engaged with at least one hose segment of the plurality of hose segments. The pressure gauge is configured to read a pressure of the fluid flowing therethrough. The first solenoid is configured to allow fluid to flow therethrough when current is applied to the first solenoid, and is configured to prevent fluid from flowing therethrough when no current is applied to the first solenoid, wherein the centrifugal fluid transfer is controlled to improve displacement output and pressure of embalming fluid entering a cadaver during an embalming process.

Systems and methods of the invention generally relate to prolonging viability of bodily tissue, especially an organ such as a lung, by adjusting pressure as needed to maintain a constant pressure within the organ even during external pressure fluctuations due, for example, to transportation of the organ in an airplane. Gas passing into and out of the organ may be conditioned to prolong tissue viability.

A body tissue preservation system for storage and preservation of body tissue, the system comprising a body tissue monitor to determine a status of the body tissue, wherein the body tissue monitor comprises: at least one sensor configured to obtain sensor data based on a plurality of measurements of the body tissue and/or the environment surrounding the body tissue; and a controller arranged to receive the sensor data from the at least one sensor, wherein the controller is configured to: detect one or more trigger events in the sensor data, wherein each trigger event comprises sensor data which satisfies a first threshold criterion; select, for each of the one or more trigger events, a window of the sensor data associated with the trigger event; identify, for each of the one or more windows, a subset of the sensor data corresponding to the selected window; determine, for each of the one or more selected windows, whether the corresponding identified subset of sensor data satisfies a second threshold criterion; determine a status of the body tissue based on identified subsets which satisfied the second threshold criterion; and provide a status output signal based on the determined status of the body tissue.

A multi-organ repairing and transferring device without interrupting a blood flow includes a first perfusion bin, a second perfusion bin and a third perfusion bin which are configured for storing respective organs. A first pump is arranged on a first pipeline, two ends of the first pipeline are connected with the first perfusion bin and the second perfusion bin respectively, a second pump is arranged on a second pipeline, two ends of the second pipeline are connected with the first perfusion bin and the second perfusion bin respectively, a third pump is arranged on a third pipeline, two ends of the third pipeline are connected with the first perfusion bin and the third perfusion bin respectively, and a fourth pump is arranged on a fourth pipeline, two ends of the fourth pipeline are connected with the first perfusion bin and the third perfusion bin respectively.

Methods, systems, and devices preserve donor organs by alternating between static cold storage and ex vivo organ perfusion. Preserving a donor organ includes refrigerating a donor organ to form a once-refrigerated donor organ, perfusing the once-refrigerated donor organ to form a once-perfused donor organ, and refrigerating the once-perfused donor organ to form a preserved, twice-refrigerated donor organ for transplantation.