Disclosed is a device for the perfusion of an organ, including: a container of fluid, containing an organ bathed in the perfusion fluid; a first path including an inlet, an outlet and a pump; and a second path including an inlet, an outlet and a pump. The “arterial” outlet of the first path has a diameter smaller than a diameter of the “portal” outlet of the second path. The device additionally includes, between the pump and the outlet of the first path and/or between the pump and the outlet of the second path, an oxygenation unit arranged to oxygenate the fluid emerging from the “arterial” outlet of the first path more than the fluid emerging from the “portal” outlet of the second path. The device can include a communication path between the first path and the second path in order to oxygenate the second path. Use in liver transplantation.

The invention is directed to a peristaltic pump (10), comprising: a flexible membrane (1) forming a at least one bladder (2) against a support, wherein each bladder is provided with one input orifice (4) which admits a fluid to the bladder (2) and one outlet orifice (5) which releases the fluid from the bladder (2); and at least one roller bearing (6) is configured to rotate about an axis (7) and to apply a compressive force against the at least one bladder (2). The peristaltic pump may be in fluid communication with a tapered jet (11).

The invention provides apheresis devices and their use for substantial removal of all types of cfDNA for treatment of various diseases and during perfusion of an organ and/or anatomical cavity, to limit the negative effects of circulating cfDNA during organ transplantation and thus improve the quality and survival of transplanted organs, and reduce unfavorable transplantation outcomes such as transplant dysfunction, ischemia-reperfusion injury, graft rejection, and organ failure.

The present disclosure includes a perfusion system (300) for preservation of an organ or vascular tissue outside the body, and associated methods. The system can include a first fluid reservoir (308), a second fluid reservoir (307), and a perfusion circuit. The perfusion circuit can include a first perfusion pathway to couple the first fluid reservoir to tissue (314), and a second perfusion pathway to couple the second fluid reservoir to the tissue. A first set of conditions can cause fluid contained in the first fluid reservoir to flow in a first antegrade direction from the first fluid reservoir through the tissue to the second fluid reservoir. A second set conditions can cause the perfusate fluid contained in the second fluid reservoir to flow in a second antegrade direction from the second fluid reservoir through the tissue to the first fluid reservoir.

The present disclosure relates to devices, systems, and methods for modeling ocular translaminar pressure gradients ex vivo. A first fluid pressure level can be applied to a first side of a wall of donor eye cup (e.g., a posterior human eye cup) to simulate intracranial pressure (ICP), for example around the optical nerve head (ONH), and a second fluid pressure level can be applied to a second side of the wall of the donor eye cup to simulate intraocular pressure (IOP). These devices, systems, and methods are unique in that they allow ex vivo modeling of dynamic changes in translaminar pressure gradients.

A method for filling and venting a device for extracorporeal blood treatment is disclosed, such as a patient module in a heart-lung machine, without attached patient. A filling liquid from a filling liquid container located higher than the device flows by gravity via a venous side of the system into a reservoir and flows onwards into a blood pump located at the lower end of the reservoir, wherein a first controllable valve (HC1) for a venting line of a filter is opened and, after the response of an upper filling level sensor in the reservoir, is closed. An upper level of the filter is positioned higher than the upper filling level sensor, and a start-stop motion of the blood pump is performed, as a result of which a stepped flooding of the filter is made providing for an advantageous de-airing of the device.

Methods of isolating cellular products, such as pancreatic islets, may be used in diabetes research and therapeutic transplantation. The methods may involve providing a donor tissue having desired cells and undesired cells, perfusing the donor tissue with a perfusion solution, developing edema during perfusion of the donor tissue to form a swelled tissue, and separating the desired cells from undesired cellular material to obtain a cellular product. The methods may also include disrupting the tissue, and separating the desired cells from undesired cellular material to obtain the cellular product. The methods may result in an increased yield of cellular product that retains sufficient functional integrity to be useful as a transplantation resource.

A repaired ex vivo organ suitable for transplantation in a human, said repaired ex vivo organ having undergone ex vivo organ perfusion for a maintenance period, wherein said organ had been assessed as being unsuitable for transplantation into a human before the maintenance period and was determined to be suitable for transplantation after the maintenance period.

Apparatus for perfusion of multiple types of organs include base unit configured to removably couple with a perfusion module for perfusing an organ. The base unit has conduits for connecting a source of a perfusate to the organ to circulate the perfusate through the organ; first and second pumps coupled to the conduits for driving circulation of the perfusate in the conduits; and a controller configured and connected for controlling the first and second pumps to regulate the circulation of the perfusate through the organ. The controller is operable to control the first and second pumps to perfuse the organ in accordance with organ specific perfusion parameters. The organ specific perfusions parameters are selected based on the type of the organ, and may be selected by an operator for at least two organ types selected from the group of heart, liver, kidney and lung.

The present application relates to methods for perfusing a biological sample in a device comprising a channel comprising a constriction wherein the method comprises flowing a liquid through the channel, sealing the channel with the biological sample and maintaining a flow of liquid within the biological sample. The application also relates to a method for assaying a substance, a device for use in perfusing a biological sample and a method of manufacturing a device.