A lyophilization nest and method of using the same is described herein. In various embodiments, the lyophilization nest is configured to support one or more receptacles each supporting one or more substances within an interior space of the lyophilization nest. The interior space may be in fluid communication with the exterior of the lyophilization nest through one or more vent holes extending through a surface of the lyophilization nest. Each of the one or more vent holes have a corresponding sealing element configured to selectively form an air-tight seal within the vent holes, such that a controlled environment may be maintained within the interior space when the ambient conditions surrounding the lyophilization nest are not lyophilization conditions. The one or more sealing elements may be operable while the lyophilization nest is positioned within a sealed lyophilizer by depressing the sealing elements into corresponding vent holes to form the air-tight seal.

A system for freezing, storing, transporting, and thawing biopharmaceutical product, the system including a stationary equipment and one or more movable pallet, each pallet being configured to carry one or more container units, each containing biopharmaceutical product, the stationary equipment including a gas circulating unit configured to cause a circulation of cold gas and/or warm gas, from a pull inlet toward a blow outlet, wherein there is provided at least a first side plenum having one or more gas conveying channel therein, configured to be fluidly coupled to the blow outlet of the gas circulating unit, the first side plenum having a plurality of outlet ports directed toward an interior volume of the pallet, wherein the gas circulates substantially in a horizontal direction in the interior volume of the pallet.

A portable cooler container is provided. The temperature control system cools a chamber of the container to transport temperature sensitive contents via the container. An electronic display screen on one of the lid and the container body selectively displays an electronic shipping label for the portable cooler container.

A portable cooler container is provided. The temperature control system cools a chamber of the container to transport temperature sensitive contents via the container. An electronic display screen on one of the lid and the container body selectively displays an electronic shipping label for the portable cooler container.

An apparatus for maintaining the temperature of an article at a temperature that is below the ambient air temperature includes an enclosure having an outer wall that defines an interior chamber for holding a volume of sealed air. An insert is disposed inside of the chamber and has a body that is made of a porous graphite foam material. A vacuum pump penetrates the outer wall and fluidly connects the sealed air in the interior chamber with the ambient air outside of the enclosure. The temperatures of the insert and article is maintained at temperatures that are below the ambient air temperature when a volume of a liquid is wicked into the pores of the porous insert and the vacuum pump is activated to reduce the pressure of a volume of sealed air within the interior chamber to a pressure that is below the vapor pressure of the liquid.

Disclosed are a carrier, a vacuumizing device, and a tissue cryopreservation system. The carrier is provided with at least one freezing chamber for loading a tissue, and a contact surface of the freezing chamber that comes into contact with the tissue is provided with a plurality of flow guide holes. The carrier may cooperate with the vacuumizing device so as to suck away excess liquid, such as a protective agent, on a surface of the tissue by vacuum, and discharge the liquid from the freezing chamber through the flow guide holes, thereby reducing the toxicity damage that may be caused by prolonged contact between the tissue and a high-concentration protective agent.

An apparatus, a system, and methods for maintaining and monitoring an excised donor heart. The apparatus comprises a first component for receiving and submerging therein an excised heart in a constantly circulating perfusate solution and a second component comprising equipment for adjusting the temperature and oxygen content of the perfusate solution. The first component comprises an integral pair of defibrillating pads. A first conduit infrastructure interconnects the first module, the second module and an aorta of the excised donor heart pushing a perfusion solution from the first module through the second module into the aorta. The second conduit infrastructure connects the first module with the right atrium and the left atrium for pushing the perfusion solution from the first module into the atria. The third conduit infrastructure connects the first module with the pulmonary artery and provides an after pressure to the flow of the perfusion solution from the pulmonary artery.

The present invention relaters to a system and methods for automated vitrification of mammalian oocytes or embryos. The system and methods enable automated processing of oocytes or embryos in vitrification solutions; robotically moving vitrification devices that carry processed cells for freezing in liquid nitrogen; automated sealing of the frozen devices; and transferring the sealed devices to an automated storage system for long-term cryopreservation.

A system for lung preservation is described. The system includes a cooler at least partially housing a cooling coil, the cooling coil including a proximal end configured to connect to an oxygen source and a distal end configured to connect to endotracheal tubing. The endotracheal tubing is in fluid communication with a conduit extending through the cooling coil. A positive end expiratory pressure valve connected to the endotracheal tubing. A method for preserving a lung and a method for preserving a lung for transportation are also described.

The present invention generally relates to thawing a cryogenically frozen sample. The systems, devices, and methods may be used to heat a sample holder, the sample holder configured to receive a sample container holding the frozen sample. A sample thaw start time may be identified by measuring a temperature of the sample container and/or a temperature of the sample. A sample thaw end time may be calculated as a function of the sample thaw start time. In some embodiments, the same thaw start time may be identified by a significant change in a first derivative of a warming curve of recorded temperature measurements. The sample end time may be calculated by adding a constant to the sample thaw start time. The constant may be the average sample thaw time per the sample container.