Technologies for rapid equilibration for water isotope analysis are disclosed. In at least one illustrative embodiment, a vaporizer may include an injection block that defines a chamber and a septum positioned over an inlet of the chamber to seal the chamber. The chamber may be configured to be fluidly coupled to a pump to develop a vacuum within the chamber, and the septum may be configured to receive a needle that is inserted into the chamber. A thermally conductive wool may be positioned within the chamber and may be configured to receive a tip of the needle.

A method of ionising a sample is provided, comprising providing a fluid sample, wherein the fluid sample contains an analyte, applying one or more pulses of acoustic energy to the fluid sample to cause a spray of the fluid sample to eject from the surface of the fluid sample, and applying an AC, RF or alternating voltage to the fluid sample using an electrode.

A secondary ultrasonic nebulisation device is disclosed comprising: a liquid sample delivery capillary; a sample receiving surface arranged for receiving a liquid sample from the capillary; and an ultrasonic transducer configured for oscillating the surface so as to nebulise the liquid sample received thereon, wherein the device is configured such that the oscillations of the surface by the ultrasonic transducer cause charged droplets and/or gas phase ions to be generated from the sample.

Mass spectrometry systems and methods including ionization devices are provided. The ionization device includes either a gas pulse valve or a piezoelectric striker. The ionization device is configured to direct force to the back of a substrate, where an analyte of interest is deposited on the front of the substrate. The impact ionizes the analyte and the ions are directed into a mass spectrometer for analysis.

An ion source for a mass spectrometer is disclosed comprising an ultrasonic transducer which focuses ultrasonic energy onto a surface of a sample fluid without directly contacting the sample fluid.

A method of ejecting a plurality of samples from a well plate includes receiving a first sample intensity prediction associated with a first sample in a first well of the well plate. A second sample intensity prediction associated with a second sample in a second well is also received. The second sample intensity prediction is less than the first sample intensity prediction. An ejection time delay value for a subsequent analysis of the first sample and the second sample is determined, based at least in part on the second sample intensity prediction. Thereafter, the first sample is acoustically ejected from the first well, and the second sample is acoustically ejected from the second well.

Provided herein are systems and methods for sampling analytes into an atmospheric pressure inlet mass spectrometer using ultrasonic nebulization-assisted atmospheric pressure chemical ionization. The systems can include a mass spectrometer having an input and an ultrasonic nebulizer chip. The ultrasonic nebulizer chip can be operatively coupled to the mass spectrometer, such that when the ultrasonic nebulizer chip nebulizes the analyte to provide a nebulized analyte, at least some of the nebulized analyte enters the input of the mass spectrometer.

A method of sampling an ejection of a sample from a liquid container includes disposing the liquid container adjacent an open port in-terface. The container includes a sampling port. The open port interface en-gages with the sampling port. The sample from the liquid container is eject-ed, through the sampling port, and into the open port interface. The sample is analyzed with a mass spectrometry device.

A method of mass spectrometry is disclosed comprising repeatedly or continuously causing first analyte sample to be released or ejected from a first sample. A determination is made as to whether or not a quality threshold such as an intensity threshold has been met or exceeded, wherein if the quality threshold has been met or exceeded then the method further comprises repeatedly or continuously causing second analyte sample to be released or ejected from a second sample.

A secondary ultrasonic nebulization device is disclosed comprising: a liquid sample delivery capillary; a sample receiving surface arranged for receiving a liquid sample from the capillary; and an ultrasonic transducer configured for oscillating the surface so as to nebulize the liquid sample received thereon, wherein the device is configured such that the oscillations of the surface by the ultrasonic transducer cause charged droplets and/or gas phase ions to be generated from the sample.