A mass spectrometer includes an ion mobility spectrometer or separator arranged upstream of a collision or fragmentation cell. Ions are separated according to their ion mobility within the ion mobility spectrometer or separator. The kinetic energy of the ions exiting the ion mobility spectrometer or separator is increased substantially linearly with time in order to optimize the fragmentation energy of ions as they enter the collision or fragmentation cell. During the time that the potential of the ion mobility spectrometer or separator is being varied, the potential of ion-optical components upstream of the ion mobility spectrometer or separator such as an ion source, ion guide, quadrupole mass filter, optional second collision or fragmentation cell and an ion trapping device are kept constant.

To provide a mass spectrometer capable of shortening the time required to obtain the mass spectrum over the wide range of the mass-to-charge ratios. A mass spectrometer includes an ionizing unit that generates ions from a sample, a mass filter that separates the ions according to a mass-to-charge ratio, and a detecting unit that detects the ions separated by the mass filter. The mass spectrometer further includes an ion guide that transports the ions to the mass filter, and a control unit that generates a mass spectrum and a mass chromatogram using detection signals obtained by performing a sweep control and a step control, the sweep control gradually increases a high frequency voltage to be applied to the ion guide, and the step control constantly keeps the high frequency voltage.

A method for multiple transition monitoring of at least one analyte in a sample using a quadrupole mass analyzer is provided and comprises at least one voltage application step, wherein a direct current (DC) voltage and a radio frequency (AC) voltage are applied between two pairs of electrodes of at least one mass filter of the analyzer, wherein the AC voltage has an amplitude V.sub.AC and the DC voltage has an applicable voltage V.sub.DC, wherein a supplementary AC voltage is superimposed on top of the AC and the DC voltage, wherein an amplitude V.sub.DC of the supplementary AC voltage is $\frac{V_{\mathrm{DC},\max }}{2^{b+1}},$
wherein V.sub.DC,max is a maximum voltage output of the DC voltage and b is a bit size of at least one electronics board of the mass filter of the analyzer; and wherein at least one transition of the analyte is determined with at least one detector of the analyzer.

Systems and methods are disclosed for dynamically switching an ion guide and a TOF mass analyzer between concentrating or not concentrating ions in a targeted acquisition. Product ions are ejected from the ion guide into the TOF mass analyzer and the intensity of a known product ion is measured at two or more time steps. The ion guide initially ejects product ions using a sequential or Zeno pulsing mode that concentrates product ions with different m/z values within the TOF mass analyzer at the same time. If the intensity of the product ion is increasing and greater than a threshold intensity, the ion guide switches to a continuous or normal pulsing mode that does not concentrate ions with different m/z values in the TOF mass analyzer at the same time. Similarly, if the intensity decreases below a threshold in continuous mode, the ion guide switches back to sequential mode.

In a scan measurement in which a mass scan is repeated across a predetermined mass range, when a voltage is returned from a termination voltage of one scan to an initiation voltage for the next scan, an undershoot or other drawbacks occur to destabilize the voltage value. Therefore, an appropriate waiting time is required. Conventionally, this waiting time has been set to be constant regardless of the analysis conditions. On the other hand, in the quadrupole mass spectrometer according to the present invention, the mass difference M between the scan termination mass and the scan initiation mass is computed based on the specified mass range, and a different settling time is set in accordance with this mass difference. When the mass difference M is small and hence requires only a short voltage stabilization time, a relatively short settling time is set. This shortens the cycle period of the mass scan, which increases the temporal resolution.

The invention generally relates to mass spectrometry via frequency tagging.

The present invention discloses a method for authentication of animal species origin of leather products, which includes the following steps: Step 1: Model establishment: (1) Collect leather samples from different animal species origins, set mass spectrometric parameters, cut the surface of leather samples using a preheated electric soldering iron, and detect the resulting sample ions using the mass spectrometer; (2) Create a multivariate statistical model based on principal component analysis and linear discriminant analysis of rapid evaporative ionization mass spectrometric data and evaluate the model with cross-validation tests; Step 2: Analysis of real leather samples: detect and authenticate the identity of real leather samples based on the multivariate statistical model. The authentication method disclosed in present invention is a rapid analytical method that requires no sample pretreatment and can identify the animal species origin of leather products rapidly and accurately.

Methods of mass spectrometry comprise, for each of a plurality of sub-ranges selected from an overall m/z range, injecting a sample of precursor ions into a first ion store via an entrance aperture region, the precursor ions having m/z values within the sub-range; retaining a first portion of the sample of precursor ions within the first ion store; and ejecting a second portion of the sample of precursor ions from the first ion store via an outlet region.

Systems and methods are disclosed for dynamically switching an ion guide and a TOF mass analyzer between concentrating or not concentrating ions in a targeted acquisition. Product ions are ejected from the ion guide into the TOF mass analyzer and the intensity of a known product ion is measured at two or more time steps. The ion guide initially ejects product ions using a sequential or Zeno pulsing mode that concentrates product ions with different m/z values within the TOF mass analyzer at the same time. If the intensity of the product ion is increasing and greater than a threshold intensity, the ion guide switches to a continuous or normal pulsing mode that does not concentrate ions with different m/z values in the TOF mass analyzer at the same time. Similarly, if the intensity decreases below a threshold in continuous mode, the ion guide switches back to sequential mode.

A method for driving a linear ion trap having rod electrodes arranged so as to surround a central axis includes: an ion-introducing step for introducing ions into an ion-capturing space surrounded by the rod electrodes, and for capturing the ions by a multipole RF electric field created within the ion-capturing space; and an ion-ejecting step for creating both a DC electric field for ion extraction extending from an external area outside the ion-capturing space into the ion-capturing space through a space between two predetermined rod electrodes neighboring each other around the central axis among the plurality of rod electrodes and the multipole RF electric field, and for sequentially ejecting ions according to their m/z from the ion-capturing space toward the external area through the space between the two predetermined rod electrodes by changing at least one of the multipole RF electric field and the DC electric field.