An apparatus for making a biocompatible three-dimensional object including at least one delivery unit arranged to deliver at least one biocompatible fluid substance towards a support body having a matrix surface to obtain a coating layer of a predetermined thickness configured for coating the matrix surface. Furthermore, a handling unit is provided arranged to provide a relative movement according to at least 3 degrees of freedom between the support body and each delivery unit. The support body is arranged to be coated by the delivered biocompatible fluid substance, in order to obtain a three-dimensional object having an object surface copying the matrix surface of the support body.

The artificial blood vessel comprises a cortex layer, a fibroblast layer, a smooth muscle cell layer, an endothelial cell layer and an inner cavity. According to the artificial blood vessel, the endothelial layer, the smooth muscle cell layer, the fibroblast layer and the cortex layer are orderly arranged in a three-dimensional space by utilizing integrated technologies of plasma spraying, electrospraying, electrospining, intra-mold pouring and 3D printing; anticoagulant activity of the artificial blood vessel is enhanced by adopting an anticoagulation factor; step-by-step induced differentiation of stem cells in the artificial blood vessel is realized by adopting a growth factor controlled release method; and the artificial blood vessel is cultured by a pulsatile reactor, so that the artificial blood vessel structurally and functionally simulates natural animal blood vessels and provides a corresponding substitute for vascular transplantation and repair.

The invention relates to a method for producing a storable molded body made of bacterial cellulose and a molded body produced according to the method. A preferred method includes providing a molded body made of bacterial cellulose. Optionally, mechanically pressing the entire molded body or parts of the molded body at temperatures in the range of 10° C. to 100° C. and pressures in the range of 0.01 to 1 MPa for a pressing time of 10-200 min. Treating the molded body with a solution of 20% by weight to 50% by weight of glycerol and 50% by weight to 80% by weight of a C1-C3-alcohol/water mixture. Drying the treated molded body.

Embodiments of the present disclosure are directed to apparatuses and methods for fabricating tubular structures from a combination of fibrous materials for use in, for example, tissue engineering scaffold applications. These materials may also be useful in other biological or non-biological applications in which such tubular fibrous structures may be applicable, examples including conventional medical devices, filters, fiber optics, cable wraps, geotextiles, batteries, fuel cells, armor, and other diverse applications.

The present invention provides a reparation method of a tubular vascular graft, (a) immersing a tubular scaffold in a first light sensitivity gelatin solution, and irradiate the tubular scaffold by a first time period, to let surface of the tubular scaffold form a base layer; (b) immersing the tubular scaffold of the step (a) in a chitin gelatin solution, when the surface of the base layer form a film, then immersing the tubular scaffold into a sodium hydroxide solution to generate a middle layer of the surface of the base layer; (c) immersing the tubular scaffold of the step (b) in a second light sensitivity gelatin solution, wherein the second light sensitivity gelatin solution comprises a cell, the tubular scaffold is irradiated by a second time period to form a surface layer of the middle layer; (d) until the cell forms a tubular structure of the surface layer by the cell in the tubular scaffold of the step (c), heating the tubular scaffold by a temperature to solve the base layer into a solution, pulling out the tubular scaffold to get an artificial blood vessels.

Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

An occlusion device that includes an inner embolic element with a proximal section and a distal section, wherein the distal section has a first stiffness and the proximal section has a second stiffness. An expandable mesh is included that is capable of being transformed between a collapsed position and an expanded position, wherein the expandable mesh is disposed over a portion of the proximal section of the inner embolic device and the first stiffness is greater than the second stiffness.

A blood vessel dissecting device is disclosed, which includes a dissecting device configured to be inserted into a living body along a blood vessel to dissect tissue surrounding the vessel from tissue surrounding the vessel in a direction along a longitudinal extent of the blood vessel; and a cutting device configured to fee inserted into the living body along the longitudinal extent of the blood vessel to cut the tissue surrounding the blood vessel so the direction of longitudinal extent of the blood vessel.

The present invention provides a connective tissue body formation substrate which can form a film-like connective tissue having a desired thickness and both surfaces in a desired surface condition without prolonging the time required for formation of the connective tissue. Specifically, two tissue formation surfaces 2a and 2b are faced with each other with a tissue formation space 3 being interposed therebetween. A slit 9 is formed in the tissue formation surface 2b so that the tissue formation space 3 communicates with an outside of the substrate. A connective tissue body formation substrate 1 is installed in an environment where a biological tissue material is present. A connective tissue intrudes into the tissue formation space 3 from the slit 9. Both surfaces of the film-like connective tissue are formed so as to match the substrate surface.

This disclosure describes decellularized, biologically-engineered tubular grafts and methods of making and using such decellularized, biologically-engineered tubular grafts.