The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.

A sequencing manifold for the purpose of supplying control and supply services of pre-determined temporal sequences to fluid processing assemblies is provided. The functioning of this sequencing manifold requires that translation be applied to the sequencing ports. Actuator mechanisms may supply such translation as either continuous motion or as a series of stepwise motions. Actuator mechanism can be obtained that rely on only mechanical means without the need for a source of electricity. With such actuators, it becomes feasible to conduct the operations of fluid processing assemblies in remote and primitive locations that lack a source of electricity. One skilled in the mechanical arts can provide various actuator mechanisms to meet these requirements.

A fluid purification system has cells whose purifying capability can be regenerated. Some of the cells are arranged in series to reach a high level of purification. An automatic valve network is controlled to cycle the cells in a way that levels the loads on each, thereby maximizing the service interval for replacing expired cells, enabling all of the cells to be replaced at the same time after having each contributing approximately equally to the purification load, and operated such that at any one time, at least one cell is regenerated so as to enable continuous up-time.

A dispensing unit having a circulation system for circulating a fluid in the dispensing unit is provided. The circulation system comprises, a circulation circuit, and a mechanism configured to circulate the fluid in the circulation circuit. By circulating the fluid, the fluid is counteracted from crystallizing. A method for circulating a fluid in a dispensing unit is also provided.

The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.

An assembly for use with a water meter includes: a housing including a meter portion integrally formed with a valve portion; and a valve positioned in the valve portion and in sealable communication with an inner surface of the housing, the valve defining a valve inlet portion and a valve outlet portion, the valve inlet portion defining a vertical portion and separated from the valve outlet portion by a top edge portion defined in the vertical portion, the valve inlet portion sealable from the valve outlet portion by a diaphragm assembly of the valve, the diaphragm assembly defining a water leak passthrough configured to allow passage of water from a first side of the diaphragm assembly to a second side of the diaphragm assembly opposite from the first side.

A fluid purification system has cells whose purifying capability can be regenerated. Some of the cells are arranged in series to reach a high level of purification. An automatic valve network is controlled to cycle the cells in a way that levels the loads on each, thereby maximizing the service interval for replacing expired cells, enabling all of the cells to be replaced at the same time after having each contributing approximately equally to the purification load, and operated such that at any one time, at least one cell is regenerated so as to enable continuous up-time.

A system that fills multiple containers includes a filter with an inlet port and multiple outlet ports. The outlet ports are pre-attached to containers by respective filling lines of each container. Each container has an interior and each of the respective filling lines is connected to a respective container interior. All of the respective filling lines are sealed to the outlet ports and the containers such that the container interiors are isolated from an external environment except the inlet port, via the filter, forming a combined interior volume which is sterile. The system can produce batches of multiple sterile containers with sterile medicament in the containers.

A system for cleaning a dairy animal milker unit and applying dip to a dairy animal, the system includes a main control, an air supply, a water supply, a backflush fluid supply, a dip supply, a stall control for receiving the air, water, backflush fluid and dip supplies, and a safety valve that is adjacent to a downstream portion of the milker unit to control backflush and dip fluids being fed to the milker unit.

A system for cleaning a dairy animal milker unit and applying dip to a dairy animal, the system includes a main control, an air supply, a water supply, a backflush fluid supply, a dip supply, a stall control for receiving the air, water, backflush fluid and dip supplies, and a safety valve that is adjacent to a downstream portion of the milker unit to control backflush and dip fluids being fed to the milker unit.