Provided are a liposome composition which has a practically required long-term preservation stability, and which has a release rate of a drug on the order of several tens of hours due to releasability of a drug being able to be suitably controlled by rendering an inner water phase hyper-osmotic; and a method for producing the same. According to the present invention, it is possible to provide a liposome composition, including liposomes each of which has an inner water phase and an aqueous solution which constitutes an outer water phase and in which the liposomes are dispersed, in which the content of cholesterols is 10 mol % to 35 mol % with respect to the total amount of lipid components in the liposome composition, and each of the liposomes encapsulates a drug in a dissolved state, and an osmotic pressure of the inner water phase is 2-fold to 8-fold relative to the osmotic pressure of the outer water phase.

Disclosed herein is a particle comprising a first effector element covalently bonded to a nanoparticle via a first orthogonal moiety and a second effector element covalently bonded to the nanoparticle via a second orthogonal moiety, wherein the first orthogonal moiety and the second orthogonal moiety have different chemical structures. The first effector element and the second effector element may be, for example, a targeting agent, a therapeutic agent, and a diagnostic agent. In certain embodiments, the nanoparticle is a liposome. Methods for preparing functionalized nanoparticles, including functionalized liposomes, are also described.

The present disclosure is concerned with unilamellar liposomes and methods of making same. The disclosed unilamellar liposomes can be useful in, for example, delivering a cargo molecule (e.g., a nucleic acid) and also in inducing an immune response. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Certain embodiments are directed to methods for loading a liposome-containing alginate microsphere with an agent when the liposome-containing microsphere has been formed prior to loading of the liposome.

Therapeutic compositions are disclosed which contain a therapeutic agent and a bile acid or bile acid conjugate. The compositions can be absorbed via enterohepatic circulation. The compositions include a cationic moiety and an anionic polymer, which are coupled through electrostatic interactions. The therapeutic compositions can be used for the treatment of diseases or disorders.

According to the present disclosure, a liposomal formulation comprising at least one uncharged phospholipid without cholesterol and at least one pharmaceutical agent, wherein the at least one uncharged phospholipid forms one or more lipid bilayers without cholesterol encapsulating the at least one pharmaceutical agent, is provided. The use(s) of such a liposomal formulation and method of producing such a liposomal formulation are also provided.

The present invention provides liposome compositions containing substituted ammonium and/or polyanion, and optionally with a desired therapeutic or imaging entity. The present invention also provide methods of making the liposome compositions provided by the present invention.

Therapeutic compositions are disclosed which contain a therapeutic agent and a bile acid or bile acid conjugate. The compositions can be absorbed via enterohepatic circulation. The compositions include a cationic moiety and an anionic polymer, which are coupled through electrostatic interactions. The therapeutic compositions can be used for the treatment of diseases or disorders.

Methods of loading extracellular vesicles with payload molecules via homogenization are disclosed herein.

A nanocarrier including a silica body having a surface and defining a plurality of pores that are suitable to receive molecules therein is described. The nanocarrier also includes a lipid bilayer coating the surface, and a cargo-trapping agent within the phospholipid bilayer. The phospholipid bilayer stably seals the plurality of pores. The cargo-trapping reagent can be selected to interact with a desired cargo, such as a drug.