Disclosed herein is a method for the preparation of an amorphous solid dispersion (ASD) comprising the steps of providing an aqueous suspension comprising nano-particles having a solubility in the aqueous suspension of less than 10 g/1, water and at least one polymer, nano-dry-melting of the aqueous suspension comprising nano-particles for a time span between 0.1 seconds and 300 seconds, at a temperature between a) 20 K below the glass transition temperature as determined with DSC according to DIN EN ISO 11357-2 at a heating rate of 10 K/min of the solid components of the aqueous suspension comprising nano-particles or 20 K below the glass transition temperature of the at least one polymer, depending on what is higher, b) the decomposition temperature of the at least one polymer or the decomposition temperature of the nano-particles, depending which is lowerto form the amorphous solid dispersion.

Provided herein are methods of preparing EVs, e.g., exosomes, associated with or encapsulated various cyclic dinucleotides, including STING agonists. Also provided herein are methods of loading EVs, e.g., exosomes, with various cyclic dinucleotides, including STING agonists.

Provided is a method for producing particles including: preparing a dispersion liquid with Liquid A and Liquid B, where Liquid A is a solution containing a physiologically active substance, and Liquid B is a solution containing a base material and a surfactant; and forming particles from the dispersion liquid.

Provided herein is a method of producing peptide nanoparticles comprising vortex-mixing (a) a hydrophobic or amphiphilic peptide or peptide conjugate, (b) one or more lipids that are free of a water-soluble polymer, (c) one or more lipids covalently attached to a water-soluble polymer, and (d) a hydrophilic solvent, to provide peptide nanoparticles.

Provided are a nanocomposite including a nano-drug delivery system; and a ginseng extract or a ginsenoside isolated therefrom, and a preparation method thereof, in which the nanocomposite may be used for the prevention or treatment of cancer and inflammatory diseases. The metal nanocomposite of the present invention may be prepared in a uniform size without using an additional reducing agent or stabilizing agent in a significantly shortened time, as compared with known metal nanoparticles. Further, since the metal nanocomposite has high solubility in water and high targeting ability for cancer cells, it can be advantageously developed as drugs. Further, the metal nanocomposite exhibits high anti-cancer and anti-inflammatory activities, and thus may be usefully applied to prevention or treatment of cancer and inflammatory diseases. Furthermore, the metal nanocomposite exhibits anti-microbial activity, biofilm-degrading activity, and anti-coagulant activity, and thus may be applied to a variety of industrial fields.

A process can be used for preparing nano- or microparticles containing a carrier-polymer and a biologically active ingredient. The process is a solvent emulsion process involving an organic phase (OP) and an aqueous phase (AP) to form an emulsion. In the case of an oil-in-water emulsion (O/W), the organic phase (OP) contains the biologically active ingredient dissolved or dispersed therein. Alternatively, in the case of a water-in-oil emulsion (W.sub.1/O), the aqueous phase (AP) contains the biologically active ingredient dissolved or dispersed therein. The organic phase (OP) is saturated with the salt-containing aqueous phase (AP) and vice versa.

A stem cell biomimetic microparticle and methods of manufacture are provided that comprise a core nanoparticle and an outer layer disposed on the core nanoparticle, the core nanoparticle comprising at least one stem cell-derived secreted factor or a population of stem-cell derived exosomes embedded in a biocompatible polymer core nanoparticle, and an outer layer obtained from a cell membrane. Also provided is a method of treating a pathological condition of a patient by delivering to the patient a composition comprising the stem cell biomimetic microparticle comprising at least one stem cell-derived secreted factor or population of stem cell-derived exosomes embedded in a biocompatible polymer core microparticle and an outer layer derived from red blood cell membranes or platelet membranes disposed on the biocompatible polymer core microparticle.

A method of preparing a nanoparticle pharmaceutical delivery system. A nanoparticle pharmaceutical delivery system. A method of preparing a targeted nanoparticle pharmaceutical delivery system. A targeted nanoparticle pharmaceutical delivery system.

Methods for intravesical administration of a therapeutic agent including application of a photoactive nanogel to the mucosal surfaces of the bladder and/or intravesical application of cell-penetrating peptides. Photoactive nanogels may be aggregated by exposure to ultraviolet light, either in vitro or in vivo, to provide controlled or extended release of a therapeutic agent, such as an antibiotic.

The presently disclosed subject matter describes the use of fluorinated elastomer-based materials, in particular perfluoropolyether (PFPE)-based materials, in high-resolution soft or imprint lithographic applications, such as micro- and nanoscale replica molding, and the first nano-contact molding of organic materials to generate high fidelity features using an elastomeric mold. Accordingly, the presently disclosed subject matter describes a method for producing free-standing, isolated nanostructures of any shape using soft or imprint lithography technique.