Disclosed a plant-derived exosome as well as a preparation method and an application thereof in preparation of drugs or scaffolds for animal tissue regeneration therapy. The preparation method includes: soaking and infiltrating any part of a natural plant with a 2-(N-morpholine) ethanesulfonic acid buffer solution; removing a supernatant; collecting a wet treated sample; refrigerating, centrifuging and extracting the sample to obtain apoplastic fluid, wherein the soaking and infiltrating method is as follows: vacuum supply is performed within 6-24 h after soaking for 2-5 times, vacuum supply time is independently 5-15 s each time, and interval time between two adjacent times of vacuum supply is independently 10 s-1 min; and centrifuging the apoplastic fluid at an ultra-high speed to obtain the plant-derived exosome, wherein ultra-high speed centrifugation conditions are as follows: centrifugal force is not lower than 100000 g, centrifugation time is 1-7 h, and a temperature is 0-4° C.

Disclosed a plant-derived exosome as well as a preparation method and an application thereof in preparation of drugs or scaffolds for animal tissue regeneration therapy. The preparation method includes: soaking and infiltrating any part of a natural plant with a 2-(N-morpholine) ethanesulfonic acid buffer solution; removing a supernatant; collecting a wet treated sample; refrigerating, centrifuging and extracting the sample to obtain apoplastic fluid, wherein the soaking and infiltrating method is as follows: vacuum supply is performed within 6-24 h after soaking for 2-5 times, vacuum supply time is independently 5-15 s each time, and interval time between two adjacent times of vacuum supply is independently 10 s-1 min; and centrifuging the apoplastic fluid at an ultra-high speed to obtain the plant-derived exosome, wherein ultra-high speed centrifugation conditions are as follows: centrifugal force is not lower than 100000 g, centrifugation time is 1-7 h, and a temperature is 0-4° C.

This document provides dietary supplement compositions. For example, dietary supplement compositions having beta glucan, quercetin, and one or more of vitamin A, vitamin C, vitamin D, and zinc are provided herein.

This document provides dietary supplement compositions. For example, dietary supplement compositions having beta glucan, quercetin, and one or more of vitamin A, vitamin C, vitamin D, and zinc are provided herein.

An environmentally-friendly preparation method of a composition containing anti-inflammatory ingredients and use of the composition are provided. The method includes: S1. weighing a specified volume of water and adjusting the pH, adding Sophora flavescens roots, and subjecting a resulting mixture to extraction at a high temperature; S2. adjusting pH of an obtained solution in step S1, adding Glycyrrhiza inflata roots, and heating a resulting mixture to boiling for extraction; S3. adding Scutellaria baicalensis roots to an obtained boiling solution from the extraction in step S2, and subjecting a resulting mixture to extraction, filtration, and pH adjustment; S4. adding activated carbon to an obtained filtrate in step S3, and subjecting a resulting mixture to incubation and filtration; S5. adding a clarifying agent to an obtained filtrate in step S4, and subjecting a resulting mixture to standing and filtration; and S6. subjecting an obtained filtrate in step S5 to membrane separation.

An environmentally-friendly preparation method of a composition containing anti-inflammatory ingredients and use of the composition are provided. The method includes: S1. weighing a specified volume of water and adjusting the pH, adding Sophora flavescens roots, and subjecting a resulting mixture to extraction at a high temperature; S2. adjusting pH of an obtained solution in step S1, adding Glycyrrhiza inflata roots, and heating a resulting mixture to boiling for extraction; S3. adding Scutellaria baicalensis roots to an obtained boiling solution from the extraction in step S2, and subjecting a resulting mixture to extraction, filtration, and pH adjustment; S4. adding activated carbon to an obtained filtrate in step S3, and subjecting a resulting mixture to incubation and filtration; S5. adding a clarifying agent to an obtained filtrate in step S4, and subjecting a resulting mixture to standing and filtration; and S6. subjecting an obtained filtrate in step S5 to membrane separation.

An environmentally-friendly preparation method of a composition containing anti-inflammatory ingredients and use of the composition are provided. The method includes: S1. weighing a specified volume of water and adjusting the pH, adding Sophora flavescens roots, and subjecting a resulting mixture to extraction at a high temperature; S2. adjusting pH of an obtained solution in step S1, adding Glycyrrhiza inflata roots, and heating a resulting mixture to boiling for extraction; S3. adding Scutellaria baicalensis roots to an obtained boiling solution from the extraction in step S2, and subjecting a resulting mixture to extraction, filtration, and pH adjustment; S4. adding activated carbon to an obtained filtrate in step S3, and subjecting a resulting mixture to incubation and filtration; S5. adding a clarifying agent to an obtained filtrate in step S4, and subjecting a resulting mixture to standing and filtration; and S6. subjecting an obtained filtrate in step S5 to membrane separation.

Provided is a pharmaceutical composition containing, as an active ingredient, a Sophora japonica L. extract, for the prevention and treatment of neurodegenerative disorders and improvement of cognitive functions. Particularly, it was confirmed that the Sophora japonica L. extract, according to the present invention, has a significant effect in an Alzheimer's Disease-causing mouse model, through a Y-maze test and a Morris water maze test, thereby confirming that the Sophora japonica L. extract could be useful as an active ingredient in the pharmaceutical composition for the prevention and treatment of neurodegenerative disorders and the improvement of cognitive functions.

Provided is a pharmaceutical composition containing, as an active ingredient, a Sophora japonica L. extract, for the prevention and treatment of neurodegenerative disorders and improvement of cognitive functions. Particularly, it was confirmed that the Sophora japonica L. extract, according to the present invention, has a significant effect in an Alzheimer's Disease-causing mouse model, through a Y-maze test and a Morris water maze test, thereby confirming that the Sophora japonica L. extract could be useful as an active ingredient in the pharmaceutical composition for the prevention and treatment of neurodegenerative disorders and the improvement of cognitive functions.

The present invention provides novel compositions for suppressing or improving hair loss, and compositions for suppressing or improving graying. More specifically, it provides agents that stabilize type XVII collagen expression, preferably agents that suppress the degradation of type XVII collagen by ELANE inhibitors and such, and compositions comprising a DNA repair-promoting agent or a DNA damage-suppressing agent as an active ingredient. Advantageous suppressive effects of hair loss and graying can be obtained when a composition comprising as an active ingredient a suppressive agent of type XVII collagen degradation such as an ELANE inhibitor is administered to a mammal. For the ELANE inhibitors, suppressive agents of type XVII collagen degradation and DNA repair-promoting agents, low-molecular compounds, polypeptides, proteins, antibodies, or nucleic acid medicine such as antisense oligos or siRNAs may be used. A method of screening for effective substances for suppression of hair loss and graying is also provided.