Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.

A high integrity protection system includes a flow line including an inlet configured to be connected to a first source of pressure and an outlet configured to be connected to a downstream system. A first subsystem is installed on the flow line between the inlet and the outlet. A second subsystem is installed on the flow line between the inlet and the outlet, and the second subsystem is in a parallel flow configuration in relation to the first subsystem. The system includes a second source of pressure configured to be fluidically connected to the first subsystem and the second subsystem.

Assay cartridges are described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, thy reagent pill zones and the like. In certain embodiments, these cartridges are adapted to receive and analyze a sample collected on an applicator stick. Also described are kits including such cartridges and a cartridge reader configured to analyze an assay conducted using an assay cartridge.

A method and apparatus for compressing gases and supplying fuel to a gaseous fuel consuming device, such as a gaseous fueled vehicle or the like. One embodiment includes a gas compressor for compressing the gaseous fuel to an array of tanks having predetermined initial set points which are increasing for tanks in the array. One embodiment provides a selecting valve having first and second families of ports wherein the valve can be operated to select a plurality of ports from the first family to be fluidly connected with a plurality of ports with the second family, and such fluid connections can be changed by operation of the valve.

A hydraulic system includes a pump in communication with a fluid reservoir and powered by a motor. A pressure compensator is adapted to adjust a position of a variable displacement mechanism of the pump. A load sensing line is adapted to communicate a highest load sensing pressure from a plurality of valves to the pressure compensator. The pressure compensator adjusts the variable displacement mechanism of the pump based on the highest load sensing pressure for maintaining a constant pressure drop across one or more work ports in each of the plurality of valves. The plurality of valves each include a load sense port having an integrated check valve that includes a metering orifice.

A hemodialysis system is disclosed. The hemodialysis system includes a dialyzer, a saline container including saline, and a disposable set comprising a blood pumping tube fluidly connected to a first end of the dialyzer, an arterial line fluidly connected to a first end of the blood pumping tube, a venous line fluidly connected to a second end of the dialyzer, a saline line fluidly connected to the blood pumping tube and the saline container, and a dialyzer line fluidly connected to a second end of the blood pumping tube and a second end of the dialyzer. The hemodialysis system also includes a dialysis instrument comprising an arterial line clamp, a venous line clamp, and a saline valve. The saline rinses blood out of the arterial line when the venous line clamp is closed, the arterial line clamp is opened, and the saline value is opened.

A hemodialysis system is disclosed. The hemodialysis system includes a disposable set including a blood pumping tube, a fresh dialysate pumping tube, and a spent dialysate pumping tube. The hemodialysis system also includes a dialysis instrument including a blood pump head, a fresh dialysate pump head, a spent dialysate pump head, a first motor positioned and arranged to operate the blood pump head, a second motor positioned and arranged to operate the fresh dialysate pump head, and a third motor positioned and arranged to operate the spent dialysate pump head. When the disposable set is loaded into the dialysis instrument, the blood pumping tube comes into registry with the blood pump head, the fresh dialysate pumping tube comes into registry with the fresh dialysate pump head, and spent dialysate pumping tube comes into registry with the spent dialysate pump head.

A method and apparatus for compressing gases and supplying fuel to a gaseous fuel consuming device, such as a gaseous fueled vehicle or the like. One embodiment includes a gas compressor for compressing the gaseous fuel to an array of tanks having predetermined initial set points which are increasing for tanks in the array. One embodiment provides a selecting valve having first and second families of ports wherein the valve can be operated to select a plurality of ports from the first family to be fluidly connected with a plurality of ports with the second family, and such fluid connections can be changed by operation of the valve.

Described herein are various embodiments of differential air pressure systems and methods of using such systems. The differential air pressure system may comprise a chamber configured to receive a portion of a user's lower body and to create an air pressure differential upon the user's body. The differential air pressure system may further comprise a user seal that seal the pressure chamber to the user's body. The height of the user seal may be adjusted to accommodate users with various body heights.

Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.