The present invention provides a composition and/or an animal feed comprising polypeptides having muramidase activity and uses thereof.

The present invention describes an alternative approach to increase methionine (Met) production in eukaryotes, namely by the insertion of components of a sulfur-metabolic pathway in organisms where the pathway does not exist or has not clearly been identified. The invention describes methods for the use of polynucleotides that encode functional cysteine dioxygenase (CDO) alone or CDO and sulfinoalanine decarboxylase (SAD) polypeptides in plants to increase Met production. The preferred embodiment of the invention is in plants but other organisms may be used. Changes in Met availability will improve nutritional value of the crop.

The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, in particular the use of the proteases in animal feed.

The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with corn based feed and buffer o solution comprising a pepsin concentration of 9000 U/ml at pH 3, 40° C., 500 rpm for at least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is o present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feedstuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.

The present disclosure relates to the isolation, identification and characterization of novel sporeforming probiotic strain(s) with NSPase (Non-Starch Polysaccharides-active hydrolases) activity isolated from fish gut microbiota, methods and uses thereof. The sporeforming probiotic strain(s) with NSPase activity now disclosed are able of producing carbohydrate-active enzymes (CAZymes) that hydrolyse non-starch polysaccharides (NSPs) and accesses their potential as probiotics (PRO) for use in particular in aquafeeds.

Disclosed embodiments concern a composition and/or combination, and a method of administering the same as a feed, or to supplement the feed of, aquatic animals, particularly for aquaculture. Disclosed composition and/or combination embodiments may comprise glucan, silica, mineral clay, mannans, yucca, quillaja, a probiotic, and/or an adhesive agent. The adhesive agent may be selected particularly to facilitate administration to aquatic species. In certain embodiments the adhesive agent comprises an oil, such as soy oil, or a syrup, such as molasses, or combinations thereof. In some embodiments the composition and/or combination may further comprise polyphenol, an antimicrobial, and/or a vaccine. Also disclosed is a method for promoting growth and/or immune function in aquatic animals.

The invention relates to production of a yeast strain with increased mannan oligosaccharides, amino acids, and complex carbohydrates. The invention further relates to the process to propagate and ferment such yeast. The invention further relates to methods of use of the yeast as a feed additive for animals and a food product for humans.

A method of culturing an aquaculture of an aquatic animal species of interest is disclosed. The method comprises inoculating the aquaculture with bacteria of at least one genera selected from the group consisting of Pseudomonas, Limnohabitans, Janthinobacterium, Stenotrophomonas, Aeromonas, Acinetobacter and Morganella, wherein the bacteria are provided in an amount sufficient to increase the survival and/or weight of the aquatic animal, thereby culturing the aquaculture of the aquatic animal species.

A refined oil comprising at least one polyunsaturated fatty acid (PUFA), wherein the oil has a fatty acid ester of monochloropropanediol, epoxypropanol, and mixtures thereof in an amount of 10 ppm or less, and processes for producing the oil. The oil is preferably a microbial or marine oil.

The use of waste from fruit and/or vegetables extruded at 105° C. to 180° C., namely sugar beet marc and/or apple pomace and/or currant pomace and/or chokeberry pomace and/or soya pomace as an additive to increase the viscosity of foodstuffs.