Disclosed is a marking composition comprising a marker and a hydrogel. Disclosed is a process of forming a marking composition comprising mixing a marker and a hydrogel. Disclosed is an article comprising a marker, a hydrogel, and an implement. Disclosed is a kit comprising a marker, a hydrogel, and an implement, wherein the marker and hydrogel are combined. Disclosed is a method for marking a portion of the brain in a mammal comprising: (a) removing a portion of a mammal's skull to expose a portion of the brain matter; and (b) contacting the exposed portion of the brain with a marking composition. Disclosed is a method for recording electrical activity in a brain, comprising: (a) removing a portion of the skull, thereby exposing a portion of a brain; (b) inserting an electrode into the brain; and (c) recording electrical activity via the electrode, wherein the electrode is at least partially coated with a marking composition.

The present disclosure provides polymerizable luminescent dyes useful for incorporation into polymers. The dyes and the polymers can be used in sensing and imaging applications, for example, to provide accurate and optionally long term measurements of glucose in vivo. The present disclosure also provides sensors including the polymers described herein. The sensors can be implanted into a tissue of a subject and used for long-term or short-term continuous and semi-continuous collection of data of various biochemical analytes, optionally without the use of implantable hardware of any type and/or enzymatic and electrochemical detection methods.

Shown and described is a composition and a method to prepare a dopant-free photoluminescent hydrogel with synthetic polymers are disclosed. The hydrogel can be synthesized in one embodiment by incorporating an amino acid to a citric acid based polyester oligomer followed by multiple crosslinking group functionalization through a transesterification reaction using an enzyme such as Candida antarctica Lipase B (CALB) as a catalyst. The hydrogels are injectable, degradable, and their mechanical and photoluminescent properties are tunable. An in vivo study shows that the hydrogel emits strong fluorescence under visible light excitation and can completely degrade over time.

At least one microneedle comprises a hydrogel material that includes a substance that fluoresces when the substance interacts with an analyte. A magnitude of the fluorescence varies as a function of the concentration of the analyte. During use, the hydrogel material is illuminated with illumination light in a first wavelength range while the hydrogel material interfaces with the dermal interstitial fluid layer of a subject, and a photosensor generates an output that corresponds to an amount of light received in a second wavelength range.

Oxygen sensing luminescent dyes, polymers and sensors comprising these sensors and methods of using these sensors and systems are provided.

A staining composition is provided with improved staining intensity. The composition comprises a vital dye and hyaluronic acid, wherein the vital dye comprises Chicago Sky Blue and Trypan Blue. The staining composition is used to stain the lens capsule during cataract surgery and provides sufficient contrast such that no air bubble needs to be injected in the anterior chamber during cataract surgery.

The present disclosure provides compositions and methods for real-time oxygen measurements. More particularly, the present disclosure relates to oxygen-sensing compositions including a metalloporphyrin compound.

Provided herein is a biomaterial comprising a sensor system comprising a donor fluorophore linked to a target binding moiety (TBM) and an acceptor molecule linked to a TBM, wherein, when the TBM linked to the donor fluorophore and the TBM linked to the acceptor molecule binds to a target, a resonance energy transfer (RET; e.g., Forster (or Fluorescence) resonance energy transfer (FRET), bioluminescent resonance energy transfer (BRET), chemiluminescent resonance energy transfer (CRET), or a combination thereof) from the donor fluorophore to the acceptor molecule occurs and a detectable signal is produced. An medical device, e.g., an implant, comprising the presently disclosed biomaterial comprising a sensor system is further provided. Related medical devices and solid supports are furthermore provided herein. Use of the biomaterials and medical devices in methods of determining a level of expression of a gene, an RNA, or a protein, is additionally provided.

The present disclosure provides polymerizable luminescent dyes useful for incorporation into polymers. The dyes and the polymers can be used in sensing and imaging applications, for example, to provide accurate and optionally long term measurements of glucose in vivo. The present disclosure also provides sensors including the polymers described herein. The sensors can be implanted into a tissue of a subject and used for long-term or short-term continuous and semi-continuous collection of data of various biochemical analytes, optionally without the use of implantable hardware of any type and/or enzymatic and electrochemical detection methods.

Embodiments described herein relate to compounds for the detection of wounds, e.g., chronic wounds or infected wounds, including compositions, substrates, kits, dressing materials, and articles, and systems containing such compounds. Further embodiments relate to methods of using these compositions, kits and systems in diagnostic assays, and in the diagnosis and/or detection of chronic or infected wounds based on enzymatic conversion of specific substrates which are contained in the compositions. Additional embodiments relate to methods of characterizing wounds based on expression of a plurality of markers and using such information to treat, manage, and follow-up patients suffering from chronic or infected wounds.