The invention provides powerful methods and compositions for designing, selecting, fine-tuning and optimizing polymer nanogel and other supramolecular assemblies for various properties including, for example, particle size, density and morphology, guest loading capacity and encapsulation stability, and dynamic release control.

Oxygen sensing luminescent dyes, polymers and sensors comprising these sensors and methods of using these sensors and systems are provided.

The present disclosure describes methods of use of a composition comprising PARPi-fl to be administered to the oral cavity (e.g., via topical application to surfaces of the oral cavity) followed by imaging of the oral cavity for detection of squamous cell carcinoma of the oral cavity (e.g., in vivo, e.g., in a dental office setting or intraoperatively). The results disclosed herein show that topically applied PARPi-fl and subsequent intraoperative imaging of oral cavities can improve surgical removal of squamous cell carcinoma cells compared to healthy cells.

A nanosensor-containing polymer composition for the monitoring of physiological parameters and a method for making the composition are disclosed. The composition includes a nanosensor disposed in a crosslinked, hydrophilic polymer for transdermal application into an intradermal environment. The method involves forming a mixture including nanosensors and a crosslinked polymer precursor, and subjecting the mixture to conditions suitable for crosslinking the polymer precursor to provide a nanosensor-containing crosslinked polymer.

Glucose-sensing luminescent dyes, polymers, and sensors are provided. Additionally, systems including the sensors and methods of using these sensors and systems are provided.

The present disclosure provides bioorthogonal compositions for delivering agents in a subject. The disclosure also provides methods of producing the compositions, as well as methods of using the same.

A system and method for fluorescence imaging of tissue in vivo and in situ, e.g., for minimally invasive diagnosis of patients. A fluorescent imaging system is provided that has a dye carrier coupled to the distal end of a probe containing a fiber optics bundle, which allows for the introduction of at least one fluorescent dye therein the dye carrier into a portion of the tissue of interest of a subject or patient when the dye carrier is selectively brought into contact with the portion of the tissue of interest. The resulting fluorescence images permit the acquisition of diagnostic information on the progression of diseases at cellular/tissue level in patients.

The present invention provides a method for selective delivery of a therapeutic or diagnostic agent to a targeted organ or tissue by implanting a biocompatible solid support in the patient being linked to a first binding agent, and administering a second binding agent to the patient linked to the therapeutic or diagnostic agent, such that the therapeutic or diagnostic agent accumulates at the targeted organ or tissue.

In some aspects, the present disclosure pertains to systems for forming a crosslinked polymer composition, the systems comprising (a) an iodinated multifunctional compound comprising either a plurality of electron-rich dienophile-containing moieties or a plurality of electron-poor diene-containing moieties and (b) a multifunctional multi-arm polymer comprising either a plurality of electron-poor diene-containing moieties in the case where the iodinated multifunctional compound comprises a plurality of electron-rich dienophile-containing moieties or a plurality of electron-rich dienophile-containing moieties in the case where the iodinated multifunctional compound comprises a plurality of electron-poor diene-containing moieties, wherein the crosslinked polymer composition is formed by a cycloaddition reaction occurring between the iodinated multifunctional compound and the multifunctional multi-arm polymer. Other aspects of the present disclosure pertain to crosslinked networks formed by such systems and to methods of treatment using such systems.

A method for cryoablation of a tissue of a patient includes positioning a cryoablation gel composition adjacent to a tissue of the patient, and cryoablating the tissue and cryoablation gel composition with a cryoprobe. The cryoablation gel composition includes a polymer carrier and a thermal accelerant bound to the polymer carrier. The cryoablation gel composition may include a therapeutic drug. Cryoablating the tissue and the cryoablation gel composition can achieve a lethal isotherm at temperatures between ?30 and ?50? C. in a shorter duration of time than when compared to cryoablating the tissue without the cryoablation gel composition. Cryoablating the tissue and cryoablation gel composition can achieve a larger treatment volume in the tissue than when compared to cryoablating the tissue without the cryoablation gel composition. Furthermore, cryoablating the tissue and cryoablation gel plus drug composition can release the drug to treat the cryoablated cells with the therapeutic drug.