Methods and compositions for the prevention or reduction of platelet transfusion associated complications are provided. The subject methods include modifying donor whole blood or platelets prior to transfusion to prevent or reduce alloimmune platelet refractoriness.

Methods and apparatuses for inactivating RNA and DNA within viruses situated on medical instruments. Through the use of photochemicals and photoactivation by monochromatic light, medical instruments can be rendered safer.

A solution of methylene blue having a concentration of 0.01-0.02% by means of interaction with light in the emission spectrum of monochromatic emitters having wavelengths ranging between 582 and 592 nm or between 658 and 662 nm and an overall light output of at least 280 lumens (lm), wherein the instruments are kept in said solution for about 90 minutes.

A method for reducing hemolysis and microparticle formation during storage of pathogen reduced blood. Oxygen reduced blood compositions comprising SAGM and riboflavin having reduced hemolysis. Oxygen reduced blood compositions comprising SAGM and riboflavin having reduced microparticles. Oxygen and pathogen reduced blood compositions comprising CPAD and riboflavin having reduced hemolysis. Oxygen and pathogen reduced blood compositions comprising SAGM and riboflavin having reduced microparticles.

A photosensitizer formulation can be disposed on or in a mesh; net; netting; screen; curtain of strands, fibers, or monofilaments; substrate, personal protective gear, mask, or any other suitable object. The photosensitizer formulation, when in contact with molecular oxygen and activated by light or ultrasound, produces microbicidal singlet oxygen. A variety of different arrangements and applications are described. For example, an air flow device may also be included to generate a flow of air through or over the photosensitizer formulation. A fluorescent formulation may be included to monitor photobleaching. The photosensitizer formulation may be disposed in a concentration gradient to generate antigenic particles by damaging or destroying microbes.

A method of disinfection of a surface of a subject of harmful microorganisms including pathogenic bacteria and viruses upon visible light irradiation using a hybridized fluorescent silk is provided. The method includes placing a predetermined quantity of the hybridized fluorescent silk i) directly on to a skin surface of a subject; or ii) on a medium and then placing the medium on the skin surface of the subject. The method further includes applying light in the visible spectrum for a predetermined amount of time to the placed quantity of hybridized fluorescent silk, wherein the hybridized fluorescent silk is one of KillerRed, SuperNova, KillerOrange, Dronpa, TurboGFP, mCherry, or any combination thereof.

A method of inactivating harmful microorganisms of a filtration medium including pathogenic bacteria and viruses is disclosed which includes placing a predetermined quantity of a hybridized fluorescent silk on to a filtration medium, applying light for a predetermined amount of time to the placed quantity of the hybridized fluorescent silk, and passing a fluid through the medium, wherein the fluid is one of substantially air or substantially water, wherein the hybridized fluorescent silk is one of KillerRed, SuperNova, KillerOrange, Dronpa, TurboGFP, mCherry, or any combination thereof.

A method for reducing hemolysis and microparticle formation during storage of pathogen reduced blood. Oxygen reduced blood compositions comprising SAGM and riboflavin having reduced hemolysis. Oxygen reduced blood compositions comprising SAGM and riboflavin having reduced microparticles. Oxygen and pathogen reduced blood compositions comprising CPAD and riboflavin having reduced hemolysis. Oxygen and pathogen reduced blood compositions comprising SAGM and riboflavin having reduced microparticles.

Disinfecting particulate material includes combining the particulate material with a photocatalyst to yield a mixture, irradiating the mixture for a length of time with pulses from a light source having broadband emission spectrum between 190 nm-1100 nm to yield an irradiated mixture, and separating the photocatalyst from the disinfected mixture to yield the disinfected particulate matter. Irradiating the mixture inactivates microorganisms in the mixture to yield a disinfected mixture. A system for disinfecting particulate material includes a pulsed light source having a broadband emission spectrum in a range between about 190 nm and about 1100 nm, a chamber defining a cavity optically coupled to the pulsed light source, a humidifier, a fan, and one or more sensors. The chamber includes a conveyor configured to accept the particulate material and arranged such that the pulses emitted by the pulsed light source irradiate the particulate material on the conveyor.

Methods and apparatuses for inactivating RNA and DNA within viruses situated on medical instruments. Through the use of photochemicals and photoactivation by monochromatic light, medical instruments can be rendered safer. A solution of methylene blue having a concentration of 0.01-0.02% by means of interaction with light in the emission spectrum of monochromatic emitters having wavelengths ranging between 582 and 592 nm or between 658 and 662 nm and an overall light output of at least 280 lumens (lm), wherein the instruments are kept in said solution for about 90 minutes.

A method for reducing hemolysis and microparticle formation during storage of pathogen reduced blood. Oxygen reduced blood compositions comprising SAGM and riboflavin having reduced hemolysis. Oxygen reduced blood compositions comprising SAGM and riboflavin having reduced microparticles. Oxygen and pathogen reduced blood compositions comprising CPAD and riboflavin having reduced hemolysis. Oxygen and pathogen reduced blood compositions comprising SAGM and riboflavin having reduced microparticles.