The present invention relates to a method of preparing a three-dimensional (3D) cell composition comprising the steps of a) forming a support matrix containing oral fibroblasts suspended within the support matrix by mixing fibrinogen, a modifier and oral fibroblasts with thrombin, b) incubating the support matrix in a cell culture media for a sufficient time to allow development of a first layer of the three-dimensional cell composition, and c) seeding oral keratinocytes on a surface of the first layer and culturing the oral keratinocytes to form a second layer of the three-dimensional cell composition. In specific embodiments of the invention, the method is exemplified for the production of an artificial gingival tissue, wherein polyethylene oxide), 4-arm, succinimidyl glutarate terminated is used as the modifier. Also disclosed are uses of the 3D cell composition which include treatment of a gum disease or condition, regenerative therapy and for in vitro testing.

Described herein are cellular scaffolds comprising a cell reservoir, a guide attached to the cell reservoir constructed from one or more biodegradable polymers, and a population of folliculo-genic cells. The cellular scaffolds are useful in growing hair.

The invention disclosed herein generally relates to methods and systems for growing, expanding and/or obtaining 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity. In particular, the invention disclosed herein relates to methods and systems for growing human cells having SOX9 protein activity and SOX2+ protein activity in vitro, and for promoting pluripotent stem cell derived ventral-anterior foregut spheroid tissue into 3-dimensional lung-like epithelium comprising cells having SOX9 protein activity and SOX2+ protein activity.

Disclosed is a method for preparing an implant including a culture of cells on a membrane, the method including steps that consist of: securing the membrane to a mounting; placing the membrane in a recess of a housing providing two spaces having adjusted heights, above and below the membrane; injecting, into the spaces, a liquid capable of transforming into gel at a transport temperature lower than an injection temperature of the liquid; and bringing the housing to the transport temperature, so as to form an implant including the membrane and two layers of gel having adjusted thicknesses, on two opposite faces of the membrane, respectively.

Methods of producing and methods of treatment using a nipple areolar complex extracellular matrix. A method for processing tissue comprises harvesting a human nipple areolar complex tissue, treating the tissue with a stabilizing medium, wherein the medium stabilizes the tissue during transport, decellularizing the tissue, and sterilizing the tissue to form a human nipple areolar complex extracellular matrix scaffold (ECMS). A method of treating an individual with a need for a nipple reconstruction comprises forming an ECMS and applying the ECMS to the individual for nipple areolar regeneration.

Provided herein are novel methods and compositions utilizing adipose tissue-derived stromal stem cells for treating fistulae.

The present disclosure relates to a composition and methods for preparing a tissue-derived, dissolved extracellular matrix. In implementations, the extracellular matrix composition includes components native to the cornea including liquefied corneal tissue. The extracellular matrix composition can be prepared by dissolving extracted corneas in an aqueous solution of inorganic base. The resulting solution is then dialyzed to achieve a physiological pH. In some implementations, the extracellular matrix composition can be seeded and/or chemically cross-linked to form a gel matrix. The extracellular matrix and/or the gel matrix can be seeded with a desired cell type (e.g., endothelial cells, epithelial cells, stromal keratocytes, etc.) to stimulate cell proliferation and regeneration.

Provided herein are novel methods and composition utilizing adipose tissue-derived stromal stem cells for treating fistulae.

Methods of producing and methods of treatment using a nipple areolar complex extracellular matrix. A method for processing tissue comprises harvesting a human nipple areolar complex tissue, treating the tissue with a stabilizing medium, wherein the medium stabilizes the tissue during transport, decellularizing the tissue, and sterilizing the tissue to form a human nipple areolar complex extracellular matrix scaffold (ECMS). A method of treating an individual with a need for a nipple reconstruction comprises forming an ECMS and applying the ECMS to the individual for nipple areolar regeneration.

The invention concerns an intraocular lens implant for placement into an intracapsular space of a lens capsule of an eye and a composition, kit and methods related to the lens implant. The lens implant is designed for a placement into a posterior portion of the intracapsular space after removal of a native lens body and has a convex posterior surface. The lens implant is formed of one part and is manufactured from a suitable transparent non-structural cellular material. This keeps an anterior portion of the intracapsular space free of the implant which is dimensioned to comprise at most 40% of a volume of the native lens body.