Methods are disclosed for forming tissue engineered, tubular gut-sphincter complexes from intestinal circular smooth muscle cells, sphincteric smooth muscle cells and enteric neural progenitor cells. The intestinal smooth muscle cells and neural progenitor cells can be seeded on a mold with a surface texture that induces longitudinal alignment of the intestinal smooth muscle cells and co-cultured until an innervated aligned smooth muscle sheet is obtained. The innervated smooth muscle sheet can then be wrapped around a tubular scaffold to form an intestinal tissue construct. Additionally, the sphincteric smooth muscle cells and additional enteric neural progenitor cells can be mixed in a biocompatiable gel solution, and the gel and admixed cells applied to a mold having a central post such that the sphinteric smooth muscle and neural progenitor cells can be cultured to form an innervated sphincter construct around the mold post. This innervated sphincter construct can also be transferred to the tubular scaffold such that the intestinal tissue construct and sphincter construct contact each other, and the resulting combined sphincter and intestinal tissue constructs can be further cultured about the scaffold until a unified tubular gut-sphincter complex is obtained.

A method of producing a cardiomyocyte population, the method comprising culturing a cell population containing cardiomyocytes seeded at a cell density of 0.1×10.sup.5 to 5.0×10.sup.5 cells/cm.sup.2 in a culture solution to proliferate the cardiomyocytes.

Provided is a tissue gel for transplantation and a method of preparing the same, including: a process (a) of preparing a composition containing a decellularized extracellular matrix; a process (b) of maintaining a tensile state by applying a tensile force to a stretching device made of an elastic polymer material; and a process (c) of injecting the composition prepared in the process (a) into the stretching device maintained in the tensile state in the process (b), allowing the composition to crosslink, and then removing the tensile force to align fibrils in the decellularized extracellular matrix in one direction.

Sheet structures for regenerating damaged or diseased mammalian tissue that are formed from acellular dermal mammalian tissue. The acellular dermal mammalian tissue includes extracellular matrix (ECM) and a supplemental bioactive component. The supplemental bioactive component can comprise a nucleic acid, such as RNA, and/or a cell, such as an embryonic stem cell. The sheet structures induce angiogenesis and, thereby, regeneration of new mammalian tissue.

A method for regeneration or repair of an infarcted myocardium including an infarcted region in an animal comprising injecting into the animal a composition including one or more gold nanostructures is disclosed.

Tissue compositions and methods of preparation thereof are provided. The tissue compositions can be used to treat or regenerate muscle tissue. The compositions can be configured to provide increased strength compared to other muscle matrices.

The present invention provides methods of regenerating muscle tissue and methods of treating volumetric muscle loss comprising administering decellularized fetal matrix scaffold. Also provided are methods for treating soft tissue injury and improving reconstructive surgery.

A method for treating damaged or diseased mammalian tissue that includes the steps of (i) providing a sheet structure formed from acellular dermal mammalian tissue that includes extracellular matrix (ECM) and a supplemental bioactive component, such as a nucleic acid, and/or a cell, and (ii) delivering the sheet structure to the damaged or diseased mammalian tissue, wherein the sheet structure induces angiogenesis and, thereby, regeneration of new mammalian tissue.

A composition for regeneration of skeletal muscle includes a nerve cell secretome or an isolate thereof. An implantable therapeutic material for regeneration of skeletal muscle includes a hydrogel and nerve cells encapsulated within the hydrogel, wherein at least some of the nerve cells are living. A method of regenerating skeletal muscle includes applying the composition or implanting the implantable material adjacent to skeletal muscle myoblasts and/or myogenic cells to regenerate skeletal muscle therefrom.

Disclosed herein are contractile cell constructs, methods for using them to treat disease, and methods for making them.