This invention relates to a lamellae tissue layer, comprising a grooved silk fibroin substrate comprising tissue-specific cells. The silk fibroin substrates provides an excellent means of controlling and culturing cell and extracellular matrix development. A multitude of lamellae tissue layers can be used to create a tissue-engineered organ, such as a tissue-engineered cornea. The tissue-engineered organ is non-immunogenic and biocompatible.

The present invention provides a preparation method of a three-dimensional construct for regenerating a cardiac muscle tissue comprising; a step of forming a three-dimensional construct by printing and crosslinking the first bioprinting composition comprising a tissue engineering construct forming solution containing decellularized extracellular matrix and a crosslinking agent, and cardiac progenitor cells, and the second bioprinting composition comprising the tissue engineering construct forming solution, mesenchymal stem cells and a vascular endothelial growth factor, to arrange the first bioprint layer and the second bioprint layer alternately; and a step of obtaining a crosslink-gelated three-dimensional construct by thermally gelating the crosslinked three-dimensional construct, and a three-dimensional construct for regenerating a cardiac muscle tissue, and the preparation method according to the present invention not only equally positions the cardiac progenitor cells in the construct but also implements a vascular network composed of vascular cells in the construct, so that the viability of cells can be maintained for a long time and the cell transfer efficiency into the myocardium can be significantly improved.

The present invention discloses a vascular bone organoid and its compression-perfusion fabricator system. The compression-perfusion fabricator system includes a compression device, a perfusion chamber, a perfusion device, and a microcomputer driver. The compression device and the perfusion device are controlled and adjusted through the microcomputer driver to simultaneously provide dynamic mechanical compression and perfusion stimulation. The system simulates the dynamic microenvironment within the human body. The present invention further utilizes 3D bioprinting technology to manufacture a vascular bone organoid, which is cultured in the compression-perfusion fabricator system, to mimic the growth of bone and vascular endothelial cells within the normal dynamic physiological environment of the bone marrow cavity.

A cellularized nerve regeneration graft is disclosed that includes an electrospun biodegradable polymer conduit having an exterior surface and an interior luminal space, a plurality of fibroblasts seeded to the exterior surface of the conduit, and a system filling the interior luminal space of the conduit. The system may include a hydrogel matrix or augmented hydrogel matrix and Schwann cells. The cellularized nerve regeneration graft may be used in the repair of peripheral nerve injuries. Methods of making the cellularized nerve regeneration graft are also disclosed.

The present invention aims to provide a method for suppressing differentiation of ganglion cell, amacrine cell, horizontal cell and/or bipolar cell in a neural retina tissue containing photoreceptor precursor and/or photoreceptor, and the like. A method for suppressing differentiation of a ganglion cell, an amacrine cell, a horizontal cell and/or a bipolar cell in a neural retinal tissue containing a photoreceptor precursor and/or a photoreceptor, including a step of culturing a retinal tissue comprising a neural retinal progenitor cell and in any stage between a differentiation stage immediately after emergence of a ganglion cell and a differentiation stage where emergence rate of a cone photoreceptor precursor reaches maximum in a medium containing a thyroid gland hormone signal transduction pathway agonist.

Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

This invention relates to apparatus, kits and methods for the production of biomimetic constructs by plastically compressing a gel, such as a collagen gel, in a well using a plunger, which may be porous. The apparatus, kits and methods allow biomimetic constructs to be produced in a controlled and reproducible manner and are suitable for the production of multilayered constructs.

This invention relates to a lamellae tissue layer, comprising a grooved silk fibroin substrate comprising tissue-specific cells. The silk fibroin substrates provides an excellent means of controlling and culturing cell and extracellular matrix development. A multitude of lamellae tissue layers can be used to create a tissue-engineered organ, such as a tissue-engineered cornea. The tissue-engineered organ is non-immunogenic and biocompatible.

Biodegradable photoluminescent polymer (BPLP) which comprises an oligomer synthesized from a multifunctional monomer, a diol, and an amino acid by reacting (i) a multifunctional monomer comprising citric acid or triethyl citrate with (ii) a diol to form a reaction product, and further reacting the reaction product with (iii) an amino acid, wherein the amino acid is linked as a side group to the oligomer backbone. The BPLP of the present invention poses tunable fluorescence emission characteristics and are cell-compatible and biodegradable. The BPLP can serve as both implant materials and bioimaging probes.

The invention relates to scaffold-free three dimensional nerve fibroblast constructs and method of generating the nerve fibroblast constructs. The invention also relates to methods or repairing nerve transection and replacing damaged nerve tissue using the nerve fibroblast constructs of the invention.