Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyveromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

Provided is a mobile fluid pumping system which may include a reservoir and, a pump actuated by a reversible motor. In the first configuration, the reversible motor is operated in a first direction causing a fluid to flow in a first direction and operated in a second direction causing fluid to flow in a second direction thereby providing means to fill a reservoir and then transfer fluid from the reservoir to a vessel or tank. The fluid delivery device provides means to substantially evacuate fluid from any connectors in the system thereby avoiding problems otherwise associated with remaining fluid that may freeze. Further, the device is designed to provide a closed load and unload system meeting ISO 022241.

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyveromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

A liquid chromatography system includes a pumping system with a selector valve in fluidic communication with a pump inlet. The selector valve switches between a first position, in which the selector valve fluidically couples a solvent reservoir to the pump inlet, and a second position, in which the selector valve fluidically couples a pressurized source of liquefied carbon dioxide (for example) to the pump inlet. The liquid chromatography system can perform as a HPLC system (or as an UPLC system) when the selector valve is in the first position and as a CO.sub.2-based chromatography system when the selector valve is in the second position. The selector valve can have a third position in which both the fluidic pathway between the solvent reservoir and the pump inlet and the fluidic pathway between the pressurized source and the pump inlet are blocked. This shut-off position advantageously facilitates system maintenance.

Automated fluid handling system comprising a housing and two or more fluid handling units arranged as interchangeable modular components with an external fluidics section and an internal non fluidics section, and wherein the housing comprises a liquid handling panel with two or more of component positions for receiving said interchangeable modular components such that the external fluidics section is separated from the non fluidics section by the liquid handling panel.

Provided is a mobile fluid pumping system which may include a reservoir and, a pump actuated by a reversible motor. In the first configuration, the reversible motor is operated in a first direction causing a fluid to flow in a first direction and operated in a second direction causing fluid to flow in a second direction thereby providing means to fill a reservoir and then transfer fluid from the reservoir to a vessel or tank. The fluid delivery device provides means to substantially evacuate fluid from any connectors in the system thereby avoiding problems otherwise associated with remaining fluid that may freeze. Further, the device is designed to provide a closed load and unload system meeting ISO 022241.

A system may include a first conduit configured to form a first batch of discrete volumes of aqueous fluid separated by spacing liquid disposed between consecutive volumes of aqueous fluid, the spacing liquid being immiscible with the aqueous fluid volumes; a second conduit, fluidically coupled to the first conduit, the second conduit configured to statically hold the first batch of discrete volumes of aqueous fluid; and a third conduit configured to receive the first batch of discrete volumes of aqueous fluid from the second conduit. The third conduit can be configured to transfer the discrete volumes of aqueous fluid of the first batch for downstream processing.

Provided is a mobile fluid pumping system which may include a reservoir and, a pump actuated by a reversible motor. In the first configuration, the reversible motor is operated in a first direction causing a fluid to flow in a first direction and operated in a second direction causing fluid to flow in a second direction thereby providing means to fill a reservoir and then transfer fluid from the reservoir to a vessel or tank. The fluid delivery device provides means to substantially evacuate fluid from any connectors in the system thereby avoiding problems otherwise associated with remaining fluid that may freeze. Further, the device is designed to provide a closed load and unload system meeting ISO 022241.

Automated fluid handling system comprising a housing and two or more fluid handling units arranged as interchangeable modular components with an external fluidics section and an internal non fluidics section, and wherein the housing comprises a liquid handling panel with two or more of component positions for receiving said interchangeable modular components such that the external fluidics section is separated from the non fluidics section by the liquid handling panel.

A pump assembly for a liquid trap of a vehicle fuel tank assembly includes a pump configured to couple to the liquid trap and selectively drain liquid therefrom, a sensor configured to monitor an inductive signature of the pump, and a controller programmed to operate the pump and monitor the inductive signature to determine if liquid is present in the liquid trap based on the monitored inductive signature.