Microfluidic devices and methods for using the same are provided. Aspects of the invention include microfluidic devices that include a separation medium and a pan-capture binding medium. The microfluidic devices are configured to subject a sample to two or more directionally distinct electric fields. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.

A pipette tip device for use in dispersive SPE. The device includes a pipette tip having a lower barrier, loose sorbent that is freely moveable during the extraction process, and a baffle system that is shaped to disrupt the flow of liquid sample that is aspirated into the pipette tip. The baffle system includes an insert that may be separate from or monolithic with the interior of the pipette tip.

A method of processing a sample in a receptacle comprising a plurality of chambers. Each of the chambers is connected to at least one other chamber by a portal and at least a first one of the chambers is formed of a flexible material. The method includes the steps of causing gas bubbles contained in the first chamber to accumulate in a portion of the first chamber, applying a compressive external force to the first chamber to cause some or all of the liquid contents of the first chamber to flow into an interconnected second chamber through a portal connecting the first and second chambers; and preventing the gas bubbles accumulated in a portion of the first chamber from flowing through the portal into the second chamber.

The invention consists of a method for determining Total Dietary Fiber (TDF) and its sub-fractions, Insoluble Dietary Fiber (IDF) and Soluble Dietary Fiber (SDF) in food and feed samples which utilizes flexible reaction/filtration containers that can be divided into one or more sections for capturing the IDF and SDF fractions separately or for capturing TDF in its entirety. Each container is fashioned as a bag that can be temporarily sealed in multiple locations to create multiple sections and is made of non-porous and porous material. Use of these containers eliminates the need for problematic transfers of mixtures from beaker to filter, and vastly improves the filtration process.

An automated dispersive liquid-liquid microextraction method of detecting and quanta N-nitrosamines in an aqueous sample. The method includes (a) extracting an aqueous solution containing the N-nitrosamines by mixing an extraction solvent and a dispersive solvent with the aqueous solution, such that the N-nitrosamines, or a portion thereof, re-distribute from the aqueous solution to the extraction solvent, (b) permitting the resulting mixture in (a) to form a two-phase mixture containing an aqueous phase comprising containing the aqueous solution with reduced amounts of the N-nitrosamines and an organic phase containing the extraction solvent with the N-nitrosamines extracted from the aqueous solution, (c) injecting the organic phase, or a portion thereof, into an injection port of a gas chromatograph coupled with at least one mass spectrometer, and (d) analyzing the N-nitrosamines by gas chromatography and mass spectrometry to detect and quantify the concentration of the N-nitrosamines in the aqueous solution.

A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.

A well plate includes a including a top portion, a bottom portion and a membrane disposed between the top portion and the bottom portion. The top portion defines a sample well in fluid communication with an opening defined by the membrane and in fluid communication with a reservoir defined by the bottom portion. The well plate is configured to be used in a centrifugation process of a test sample including a sample material and a wash liquid. The test sample configured to be received within the sample well and the reservoir. The membrane configured to filter the wash liquid from the test sample during the centrifugation process such that the wash liquid can pass from the reservoir, through the membrane and can be captured within a collection chamber while the sample material remains within the reservoir.

The present invention relates to a method for purifying pure thioflavin T in having a step of preparing a thioflavin T solution in which crude thioflavin T is dissolved in a polar solvent, a step of bringing the thioflavin T solution into contact with a non-polar polymeric porous body, and a step of separating the thioflavin T solution after the contact from the non-polar polymeric porous body.

The invention relates to the detection of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). In a particular aspect, the invention relates to methods for detecting DHA and EPA by mass spectrometry and kits for carrying out such methods.

A method of pretreating a sample including biological particles, the method including a step of acquiring a fraction (1b) which passes through a sieve (A) having meshes of 250 to 1000 μm and does not pass through a sieve (B) having meshes of 32 to 63 μm by sieving a sample including biological particles as a detection target, and a step of adding a colloidal solution having a density of 1.10 to 2.45 g/cm.sup.3 to the fraction (1b), subjecting the resultant solution to centrifugation, and acquiring a supernatant fraction (S0) after the centrifugation.