Provided herein is a method for sterilely filtering amniotic fluid from selected caesarean sections of an individual. The amniotic fluid is first centrifuged at 5,000 to 10,000 rpm for 30 to 60 minutes and filtered through filters with about 5 to about 10 m pore size. Next, the fluid is sequentially filtered through a series of membrane filters with the pore sizes 1 m and 0.45 or/and 0.2 m. The filtrate is then aseptically transferred to and sealed in syringes or vials. The fluid is subsequently lyophilized to obtain the lyophilisate of amniotic fluid. Amniotic fluid is reconstituted by adding sterile water to the lyophilisate, and the reconstituted fluid is used for wound healing, cosmetic, orthopedic or ophthalmic applications, particularly for the treatment of dry eyes.

A device for aseptic delivery of biological material from a vial includes a tubular barrel, a filter assembly, and a dispersion assembly. The dispersion assembly is at least partially disposed within the tubular barrel. The dispersion assembly includes a dispersion element, a piston, and a one-way valve. The dispersion element is in fluid communication with the vial to disperse the biological material from the vial. The piston is disposed at the distal end of the dispersion assembly and is in sealing contact with the tubular barrel. The one-way valve forms a fluid passageway in fluid communication with the dispersion element and the tubular barrel. The one-way valve is configured to allow a flow of the dispersed biological material from the dispersion element, through the fluid passageway, and into the tubular barrel, and to prevent a flow of the dispersed biological material from the tubular barrel into the dispersion assembly.

The METHODS, APPARATUSES AND SYSTEMS FOR INSTILLING STEM CELLS AND PHARMACEUTICALS INTO THE HUMAN VENTRICULAR SYSTEM (hereinafter Ventricular Stem Cell System or VSCS) disclosed herein provide safe and effective techniques for obtaining stem cells and instilling any type of stem cell or pharmaceutical agents into the human ventricular system for treatment of various diseases, including neurodegenerative diseases such as Parkinson's, Alzheimer's, Multiple Sclerosis, and others.

The invention relates to a method and a device for separating blood using a centrifuge in order to obtain different blood fractions. A separation container may be filled with blood via a feed connection that can be aseptically connected to the container, the relevant blood fraction may be removed from the separation container via a removal connection that can be aseptically connected to the container and both the feed device and the removal device form a respective closed system during the sterile connection to the separation container. The separation container may have at least one feed connection that can be aseptically connected to the container and at least one removal connection that can be aseptically connected to the container, to which hermetically sealed feed and removal devices can be aseptically attached in a sterile manner.

The present invention relates to the field of stem cells and their use in processing ex vivo samples (e.g. blood samples). More specifically, the present invention relates to the use of mesenchymal stem cells in ECMO.

The present disclosure relates to devices and methods for the improved storage and expansion of stem cells.

A method and apparatus for delivery of cell therapies, introduced via percutaneous access to the circulation, and delivered to the site of vascular injury intervention.

The present invention relates to a biomedical device for the production, storage, traceability and administration of blood components.

The present invention relates to a method for separating lymphocytes and/or stem cells from whole blood in an automated blood separation system, wherein the quality of the collected lymphocytes and/or stem cells fractions is increased and the cell collection procedure is further automated by use of an optical sensor comprised in a detector device to measure turbidity and colour in the claimed method and in a cell separator, which can be used to perform the claimed method. The method of the invention is particularly useful to collect lymphocytes and/or stem cells fractions from whole blood, wherein the contamination of the collected cell fractions by platelets, red blood cells and granulocytes is reduced.

A method for processing biological material containing stringy tissue in a container having a tissue collector disposed in a tissue retention volume on one side of an internal filter includes washing biological material contained in the tissue retention volume with wash liquid to the tissue retention volume and allowing the wash liquid and rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect stringy material on the tissue collector.