An intracellular delivery system and method are provided. The intracellular delivery system comprises a laser-activated surface and cells positioned at a distance from the laser-activated surface. A laser provided a laser pulse that is used to porate membranes of the cells to deliver or extract cargo from the cells into a liquid surrounding the cells. The method of intracellular delivery comprises positioning a laser-activated surface at a distance from cells and applying a laser pulse from the laser to the surface to porate membranes of the cells to deliver or extract cargo from the cells into a liquid surrounding the cells.

A surgical handpiece has a connecting body with a distal end and a work tip with a hub at a proximal end. The hub is detachably connected to the connecting body by a threaded connector. The work tip has an open operating end at a distal end. This opening leads an axial channel extending through the work tip from the operating end to the hub. A radial channel extends from the axial channel in the hub to the external surface of the hub. A sleeve surrounds and is spaced from the hub. This sleeve extends to the vicinity of the operating end of the work tip, and has a first external connector in the region of the radial channel of the hub. The sleeve also has a second external connector. A seal is provided for establishing a fluid connection between the radial channel of the hub and the second external connector of the sleeve. The first external connector of the sleeve is in fluid connection with an irrigation channel between the inner surface of the sleeve and the external surface of the work tip. This irrigation channel extends to the vicinity of the operating end of the work tip for delivery of irrigation fluid to that area. The irrigation channel is generally concentric with the axial channel in the hub. Aspiration fluid is withdrawn from the open operating end of the work tip, through the axial and radial channels of the hub, the seal and the second external connector of the sleeve to an aspiration pump.

The present disclosure relates generally to the use of medical devices for the treatment of vascular conditions. In particular, the present disclosure provides devices and methods for using laser-induced pressure waves to disrupt vascular blockages. The present disclosure not only provides devices and methods for using laser-induced pressure waves to disrupt vascular blockages, but the present disclosure also provides devices and method for assisting the guidewire in penetrating an occlusion, devices and method for using a sealable valve in the tip of the balloon catheter to reduce the overall size and diameter of the balloon catheter, thereby allowing the balloon catheter to penetrate smaller size blood vessels and devices and method that use stationary light absorbing material in lieu of and/or in combination of using liquid medium that flows into a balloon for a balloon catheter. Given the persistence of coronary artery disease (CAD) and peripheral artery disease (PAD), there remains a need for improved therapeutic methods designed not only to reduce vascular blockages in the short term, but also to prevent future complications such as restenosis.

Provided is a shock wave focusing device for a shock wave ablation system for coagulating and necrosing cardiac muscle tissue that becomes a cause of arrhythmia. A shock wave generation apparatus (10) comprises: a shock wave focusing device (11) which has a concave surface (11a); an optical fiber (12) which is inserted into the shock wave focusing device (11); a tubular catheter (13) which guides the optical fiber; an enclosure (14) which constitutes a space to be filled with liquid at the tip of the optical fiber (12); and the liquid (L) which is filled into the enclosure (14). The shock wave focusing device (11) is configured from a ring-shaped coupling part (16) provided with a central hole (11b), and 16 blade parts (17) curved outward from the edge of the coupling part (16) toward the front and provided elastically to the edge of the coupling part (16), and can be folded by rotating the blade parts (17) with respect to the coupling part (16).

Methods of non-contact lithotripsy are disclosed, wherein photonic nanoparticles are delivered in the vicinity of kidney stones, e.g. within a kidney. The nanoparticles then are irradiated with non-ionizing electromagnetic radiation to activate the nanoparticles and fragment the stone. In preferred embodiments, the nanoparticles are placed effectively into contact with the kidney stone, via functionalizing the particles with ligands that will adhere to the known or anticipated chemical composition of the stone surface. A combination of a kidney stone and a photonic nanoparticle in its vicinity or adhered thereto at its surface also is disclosed.

A system and method for detecting relative location of a surgical laser fiber tip relative to a surgical laser target during a surgical laser procedure utilizes a spectrophotometer to detect radiation indicative of the relative location. For example, the detected radiation may indicate contact between the fiber tip and a stone being subjected to laser lithotripsy, so as to prompt the surgeon to withdraw the fiber tip from the stone and/or take other action to limit contact-induced erosion of the fiber tip, and to avoid saturation of the endoscope camera resulting from the flash that occurs following contact. In addition, the absence of any detected radiation by the spectrophotometer may be used to indicate that the stone is no longer present, or that the fiber tip is no longer aimed at the stone, prompting the operator to reposition the fiber and/or temporarily cease firing of the laser. The main surgical laser may be a pulsed Holmium laser, which is delivered to the target through the optical fiber together with a pulsed 532 nm aiming beam.

Provided is a surgical handpiece for providing an electromagnetic cutting blade. The handpiece, comprises a body portion having an input end and an output end, a plurality of optical fibers for receiving laser energy having a wavelength within a predetermined wavelength range, wherein the optical fibers are received in the body portion at the input end and extend to the output end, and an optical fiber transition region within the body portion for arranging the plurality of optical fibers into a predetermine cutting shape at the output end, wherein laser energy transmitted from the arranged optical fibers at the output end interact with water molecules near the surgical target to generate micro-explosions that result in a cutting effect.

The present invention provides a catheter for treating occlusions in body lumens. The catheter includes a catheter body that is fillable with fluid. An impactor is connected to the distal end of the catheter body and has a proximal end inside the catheter body and a distal end outside the catheter body. The catheter also includes a shock wave source configured to generate a shock wave, and a deflector coupled to the proximal end of the impactor in between the shock wave source and distal end of the catheter body. When the shock wave source generates a shock wave, the shock wave impinges on the deflector causing the deflector to advance in a forward direction in conjunction with the impactor such that the distal end of the impactor delivers a mechanical force to the occlusion to restore flow to the body lumen.

The presently disclosed subject matter provides techniques for inducing collagen cross-linking in human tissue, such as cartilage or cornea, without using a photosensitizer (e.g., riboflavin), by inducing ionization of the water contained in the tissue to produce free radicals that induce chemical cross-linking in the human tissue. In an embodiment, a femtosecond laser operates at sufficiently low laser pulse energy to avoid optical breakdown of tissue. In an embodiment, the femtosecond laser operates in the infrared frequency range.

Methods and apparatuses for treating a root canal in a tooth or hard and/or soft tissue within a tooth and surrounding tissues by pulsing a laser light into a reservoir, preferably after introducing liquid fluid into the reservoir, so as to disintegrate, separate, or otherwise neutralize pulp, plaque, calculus, and/or bacteria within and adjacent the fluid reservoir without elevating the temperature of any of the dentin, tooth, bones, gums, other soft tissues, other hard tissues, and any other adjacent tissue more than about 5? C.