In some embodiments the present invention provides perfusion bioreactors and cell culture scaffolds suitable for the preparation of tissue grafts, such as bone tissue grafts. In some embodiments, the perfusion bioreactors comprise a graft chamber and/or a graft chamber insert configured to hold a tissue graft having a certain shape and/or certain dimensions, and/or to allow culture of such tissue grafts under press-fit direct perfusion conditions. In some embodiments, the perfusion bioreactors comprise an equilibration chamber.

Embodiments of a reamer device for removing tissue from a patient, including a shaft, a cutting head having at least four cutting blades, and a depth-limiting flange extending radially outwardly away from the at least four cutting blades. Some embodiments of the reamer device can have a shaft, a cutting head coupled with the shaft, and a depth limiting element. The cutting head can have at least four cutting blades. In some embodiments, the depth limiting element can have a distal surface configured to contact a surface of the patient's tissue to limit a depth into the patient's tissue that the at least four cutting blades can advance to, and/or can be configured to extend radially outwardly of the at least four cutting blades so that the depth limiting element contacts a surface of the patient's tissue adjacent to a cutout created by the at least four cutting blades.

Embodiments of a reamer device for removing tissue from a patient, including a shaft, a cutting head having at least four cutting blades, and a depth-limiting flange extending radially outwardly away from the at least four cutting blades. Some embodiments of the reamer device can have a shaft, a cutting head coupled with the shaft, and a depth limiting element. The cutting head can have at least four cutting blades. In some embodiments, the depth limiting element can have a distal surface configured to contact a surface of the patient's tissue to limit a depth into the patient's tissue that the at least four cutting blades can advance to, and/or can be configured to extend radially outwardly of the at least four cutting blades so that the depth limiting element contacts a surface of the patient's tissue adjacent to a cutout created by the at least four cutting blades.