Provided are a pharmaceutical composition or a pharmaceutical composition raw material, which contains supernatant from a culture medium used during culture to induce pluripotent stem cells.

Provided is a composition including stem cell is derived from amniotic fluid of an artiodactyl animal. Also provided relates to uses of the composition for skin rejuvenation, skin treatment. promoting hair growth, and preventing hair loss, including administering an effective amount of the composition to the subject in need thereof.