A system and method of improving the condition of skin by reducing or eliminating wrinkles and hyperpigmentation is presented. The method delivers stem cells into the dermal layer of the skin to increase the number and density of fibroblasts in the dermal layer which in turn increases collagen, elastin and hyaluronic acid production to improve the appearance of the skin. The method includes constructing a scaffolding within microperforations in the skin and administering a therapeutically effective amount of stem cells to the skin which are supported on the scaffolding to remain in the microperforations for weeks thus allowing the dermal layer of skin to rebuild to a more youthful state.

The present invention relates to the fields of medicine, cell biology, molecular biology and genetics. In particular, the present invention provides methods to isolate and purify microvesicles from cell culture supernatants and biological fluids. The present invention also provides pharmaceutical compositions of microvesicles to promote or enhance wound healing, stimulate tissue regeneration, remodel scarred tissue, modulate immune reactions, alter neoplastic cell growth and/or mobility, or alter normal cell growth and/or mobility. The present invention also provides compositions of microvesicles to be used as diagnostic reagents, and methods to prepare the compositions of microvesicles.

A new preparation for regenerating tissues or healing wounds, which can be administered to a patient, which comprises serum and globin being insoluble at physiological pH, said preparation being obtained by adding said serum to said globin being insoluble at physiological pH.

This invention relates to method of improving the appearance of skin or hair. In particular, it relates to cosmetic products such as creams and serums comprising a subject's own platelet rich plasma (PRP). The invention also relates to kits that allow the user to apply the cosmetic products daily for several days.

The present invention is based on the idea of extracorporeally simulating a wound in order to obtain wound healing/regenerative growth factor mixtures. The invention provides a method for separating the growth factors that are released from cells upon coagulation (coagulation-induced signaling phase) from the growth factors that are secreted by cells under hypoxia (hypoxia-induced signaling phase), then mixing the two phases at defined ratios to obtain a novel composition. Peripheral blood is obtained and allowed to clot or centrifuged to induce clotting. The serum is removed completely or partially and replaced with fresh medium of volume equal or less than the volume of serum removed. The clot is incubated in fresh medium at a temperature of 10 to 40? C., under hypoxia (1-10% O.sub.2) for 1 to 7 days. The clot conditioned medium is then collected and mixed with the serum at a defined ratio to obtain the composition. Compositions comprising adjustable concentrations of growth factors derived from blood serum (coagulation-induced phase) and clot-conditioned medium (hypoxia-induced phase) can be added to suitable carriers, and can be topically applied or injected into a subject in order to aid wound healing, skin regeneration or rejuvenation.

Cosmetic methods providing a significant improvement over existing hair transplantation methods by providing greater hair density per hair implant, which is achieved through the process of an enhanced hair multiplication in vivo (e.g. in the scalp of a subject). The cosmetic methods also provide improved aesthetic results, particularly, more realistic and natural looking results, relative to the results obtained by traditional methods. The cosmetic methods are particularly suitable for hair transplantation in recipient areas of a subject experiencing baldness or lack of hair, optionally due to Androgenic alopecia, burn injuries, cancer chemotherapy, or other genetic or environmental factors or scarring.

The present invention is related to the field of tissue regeneration. It concerns more particularly new processes, tubes and devices for thrombin, platelet concentrate and wound healant preparations, alone or in combination with cell extracts, cell compositions and uses thereof.

There is provided a skin care formulation for transdermal administration of a component of a blood product, the formulation comprising a blood product, a transdermal carrier; and a liposomal base, wherein at least a portion of the blood product and transdermal carrier is contained within liposomes of the liposomal base.

The invention relates to a topical pharmaceutical composition for promoting hair growth and/or reducing hair loss in a human. In particular, the pharmaceutical composition comprises: (A) therapeutically effective amount of platelet component that includes a growth factor concentrate comprising platelet derived growth factor (PGDF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), and transforming growth factor- (TGF-); and (B) a permeability enhancing agent.

The invention concerns a pharmaceutical composition comprising a platelet lysate and its use to treat a wound, an anal fissure, vaginal atrophy or a wrinkle.