A method for preparing a bioactive composition containing conditioned cell culture medium is disclosed. The method comprises culturing cells of two or more eukaryotic cell line to form conditioned culture media, separating the cultured cells from the conditioned culture media, and combining conditioned culture media to form a bioactive composition. Novel bioactive compositions, formulations and their use in treating of a variety of diseases and health conditions are also disclosed.

In one aspect, a facial mask is disclosed, which comprises a polymeric matrix having a plurality of protrusions configured for penetrating into the stratum corneum of facial skin, and a plurality of exosomes that is distributed within the polymeric matrix, where the polymeric matrix is degradable when embedded within the skin and/or the physiological temperatures, e.g., 37 C. or higher, so as to release the exosomes into the skin.

The present invention provides a composition for treating or preventing skin pigmentation, which contains fibroblasts as an active ingredient, wherein the fibroblasts are those fibroblasts which are less damaged than fibroblasts localized in a site where the skin pigmentation occurs, and the composition is characterized by being intended to be applied to a site where the pigmentation occurs or a dermis tissue around the site.

The present invention relates to a composition for skin improvement, skin damage treatment, or maintenance of epidermal stemness, the composition comprising nidogen as an active ingredient. The composition comprising nidogen as an active ingredient according to an aspect exhibits effects on the epidermis and the dermis through basement membrane strengthening such as strengthening of binding between the basement membrane and the extracellular matrix so as to improve the function of the basement membrane, and proliferating of epidermal progenitor cells and maintaining of stemness of epidermal stem cells so as to improve keratin turnover and promote collagen production and binding, and thus the present invention can be utilized for skin improvement including skin barrier strengthening, suppression or prevention of hyperpigmentation, moisturization, skin elasticity, anti-aging, or tissue regeneration through wound healing.

Described herein are personalized skin care compositions including a cultured composition including a milk sample and a skin sample. and optionally an additive. Also described herein are kits and methods for making personalized skin care compositions. Also described herein are methods of treating or preventing skin conditions using personalized skin care compositions.

Pro-toothpaste compositions formulated to receive a volume of allergen solution to provide toothpastes that exhibit efficacy for oral mucosal immunotherapy (OMIT) and acceptable consumer and product stability properties, along with kits of pro-toothpaste, allergen/extracts of allergens, and optionally compounding means and/or specialized toothbrushes are provided. Toothpastes suitable and effective for OMIT and methods for managing allergic symptoms and for reducing risk of allergy in people without symptoms employing the pro-toothpastes, toothpastes, and kits of the invention are also disclosed.

Described herein are personalized skin care compositions including a cultured composition including a milk sample and a skin sample, and optionally an additive. Also described herein are kits and methods for making personalized skin care compositions. Also described herein are methods of treating or preventing skin conditions using personalized skin care compositions.

A method of preparing hair follicle-derived conditioned medium which comprises one or more bioactive factors, wherein the hair follicle is retrieved from a subject non-invasively, cultured in a culture medium for a duration sufficient for a hair follicle-derived cell to secrete the one or more bioactive factors to condition the medium. A hair follicle-derived conditioned medium comprising one or more bioactive factors secreted by a hair follicle-derived cell retrieved from a subject non-invasively. A composition comprising said conditioned medium. Use of said conditioned medium and composition for therapeutic or cosmetic treatment.