The present disclosure relates to improved supraparticles loaded with high levels of payload and methods for their production. Such supraparticles may be used in a range of therapeutic applications, for example, to improve growth or survival of cells and/or treat disease.

The present disclose generally provides pharmaceutical semi-solid hydrogels with entrapped calcium phosphate Comparison of Fitted Curves with Log Time for nanoparticles that demonstrate enhanced Preparations Demonstrating Statistical Significance drug release, retention, and esthetic properties. The hydrogels are particularly useful for topical applications of drug molecules. The present disclosure also relates to methods of administering a pharmaceutical agent by providing a pharmaceutical semi-solid hydrogel containing at least one pharmaceutical agent and administering it to a subject in need thereof.

Provided are compositions and methods for transport, monitoring the transport, and controlled release of active agents. The compositions comprise surface functionalized iron oxide nanoparticles. The iron oxide nanoparticles are surface functionalized with cucurbitril[7] macrocycles. The cavity formed by the CB[7] macrocycles can be used for storage and transport of active agents. The active agents may be imaging agents or may be therapeutic agents which can be released by applying an alternating magnetic field at desired locations.

Methods, systems, and devices are disclosed for encapsulating biological entities with preservation of their biological activity. In one aspect, a method of encapsulating a biological entity includes templating a biocompatible material onto a biological structure to form a coating structure enclosing the biological structure, the coating structure having a size in the nanometer range, in which the coated biological structure preserves its biological activity within the coating structure. In some implementations of the method, the biological structure includes a virus and the biocompatible material includes silica.

The presently disclosed subject matter provides methods for continuously generating uniform polyelectrolyte complex (PEC) nanoparticles comprising: flowing a first stream comprising one or more water-soluble polycationic polymers at a first variable flow rate into a confined chamber; flowing a second stream comprising one or more water-soluble polyanionic polymers at a second variable flow rate into the confined chamber; and impinging the first stream and the second stream in the confined chamber until the Reynolds number is from about 1,000 to about 20,000, thereby causing the one or more water-soluble polycationic polymers and the one or more water-soluble polyanionic polymers to undergo a polyelectrolyte complexation process that continuously generates PEC nanoparticles. Compositions produced from the presently disclosed methods and a device for producing the compositions are also disclosed.

The present invention relates to a composition comprising the bioactive molecules esculetin and euphorbetin, which present antitumor activity, so it may be used for the treatment and/or prevention of cancer, especially for colorectal, pancreatic and glioblastoma. Furthermore, it relates to a drug delivery system composed of nanoparticles of calcium phosphate functionalized with said bioactive molecules, preferably obtained from a Euphorbia extract. The invention also refers to the obtaining of an ethanolic extract of plant origin from defatted flour of mature seed of Euphorbia.

The invention in aspects relates to methods of treating sepsis using HDL-NP. The methods include the use of nanoparticles having a core and a lipid based shell with optimal lipids therein.

The disclosure provides nanoemulsion compositions and methods of making and using thereof to deliver a bioactive agent such as a nucleic acid to a subject. The nanoemulsion composition comprises a hydrophobic core based on inorganic nanoparticles in a lipid nanoparticle that allows imaging as well as delivering nucleic acids. Methods of using these particles for treatment and vaccination are also provided.

The invention relates to a process for the preparation of a product comprising one or more nanoparticles of calcium phosphate (CaP-NP) with negative surface charge having a ζ-potential in the range from −41.0 mV to −27.0 mV comprising the steps of: a) maintaining a mixture having a pH in the range from 7 to 10 and comprising an aqueous solution of calcium, an aqueous solution of phosphate and a solution of citrate ions at a temperature in the range from 20° C. to 40° C. for a time in the range from 30 seconds to 10 minutes; b) removing non-reacted ions from the solution of step a), thus obtaining a suspension of one or more nanoparticles of calcium phosphate (CaP-NP); c) recovering the product of one or more nanoparticles of calcium phosphate (CaP-NP) from the suspension of step b). In an advantageous embodiment, the process of the invention provides, in the mixture of step a), also an aqueous solution of one or more therapeutic/diagnostic compounds. The product of the invention may be used as a vehicle for one or more diagnostic/therapeutic compounds for the treatment of cardiovascular diseases through inhalation administration.

The disclosure provides nanoemulsion compositions and methods of making and using thereof to deliver a bioactive agent such as a nucleic acid to a subject. The nanoemulsion composition comprises a hydrophobic core based on inorganic nanoparticles in a lipid nanoparticle that allows imaging as well as delivering nucleic acids. Methods of using these particles for treatment and vaccination are also provided.