Disclosed herein are plant messenger packs (PMPs) encapsulating one or more exogenous polypeptides. Also disclosed are methods of producing a PMP comprising an exogenous polypeptide.

The present disclosure discloses a bioengineered formulation comprising: (a) a modified collagen peptide having a molecular weight in the range of 20-80 kDa, and with a degree of substitution in the range of 20-75%; and (b) a modified hyaluronic acid having a molecular weight in the range of 10-100 kDa, and with a degree of substitution in the range of 20-75%. The present disclosure also discloses the bioengineered formulation comprising stem cells, or exosomes, or combinations thereof. Further, the process for preparing the bioengineered formulation is also disclosed therein. Moreover, the formulation comprising: a) exosomes selected from the group consisting of corneal stromal stem cell derived-exosomes, primed mesenchymal stem cell derived-exosomes, and naive mesenchymal stem cell derived-exosomes; and (b) a clinically approved eye drop formulation is also provided.

The present disclosure relates to unimolecular core-shell nanoparticle, nanoclusters thereof, and platelet biomimetic nanoclusters thereof. The disclosed compositions are useful for treating a subject with a disease or condition, such as a cardiovascular disease. In a further aspect, the cardiovascular disease can be a vascular stenosis or restenosis. Also described herein are methods of making and using the unimolecular core-shell nanoparticle, nanoclusters thereof, and platelet biomimetic nanoclusters thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

The present disclosure relates generally to a method and vehicle for drug delivery. In particular, the present disclosure provides methods for delivering an agent across the blood-brain barrier and an agent delivery vehicle for same.

Human dermal fibroblasts (HDF) and melanoma (MEL) cells are used herein for synthesis of metal nanoparticles. For example, synthesis of nanoparticles of gold (Au), palladium (Pd), platinum (Pt), and bimetallic formulations of gold-palladium (AuPd) and gold-platinum (AuPt) is demonstrated with HDF and MEL using a straightforward, eco-friendly and cost-effective approach. The nanostructures are purified and used in biomedical tests, which show selective behavior. The production of nanoparticles allows for stopping of the growth of cancer cells and the ability of new healthy cells to grow on top. The production of nanoparticles with the cells allows for an environmental-resistance behavior within the cells, showing the ability to stand for extreme environmental conditions.

The present disclosure discloses embodiments of exosome compositions comprising primed mesenchymal stem cell-derived exosomes.

The present invention may comprise stabilized metal nanoparticles that are stabilized with material from gum arabic. The nanoparticles of the invention may be manufactured with an environmentally friendly method for fabricating biocompatible stabilized metal nanoparticles. The coated metal nanoparticles may be introduced in vivo to conduct therapy for humans or animals.

Provided are methods for treating or preventing myocarditis by administering extracellular vesicles, including exosomes. In some embodiments, the EVs are from bone marrow-derived mesenchymal stem (or stromal) cells. The myocarditis can be the result of viral infections such as Coxsackievirus B virus, SARS-CoV-2, or other viruses.

A nanoparticle composition of buckminsterfullerene bonded with epicatechin or a galloyl functional group such as in epigallocatechin gallate, or in tannic acid, is provided that is anti-microbial and efficacious to maintain or re-establish benign healthy cellular homeostasis. In addition, the ability to penetrate hydrophobic malignant microbes via desulfurization is promoted with the addition of phosphonate pendant groups. This further enables the composition to prevent or to treat chronic obstructive pulmonary disorder (COPD), to penetrate fungal spores, and to penetrate the hydrophobic regions of uncontrolled invasive pathological bacteria. The composition can be produced at low temperatures through reactive shear milling. Delivery methods include ingestion, topical application, topical buccal application, inhalation, or injection when used as a medicament or as a food supplement.

The present invention relates to: a photothermal agent which includes a mussel adhesive protein; and photothermal-responsive nanoparticles that generate a biocompatible gas by means of light and heat and release a drug. Nanoparticles according to the present invention exhibit a photothermal effect when near-infrared rays are applied thereto, and may be applied to trimodality therapy in which a biocompatible gas is generated by means of light and heat to induce the release of a drug.