Described herein are compounds, compositions and methods for modification of the surface of a living cell with a therapeutically relevant targeting moiety. Also described herein are methods for treating disease states, such as acute myocardial ischemia or infarction, with said compositions, in a subject.

A theranostic composition and method for determining the cell-specific potency of drugs is provided. Further, compositions and methods useful for studying the therapeutic profile of one or more drugs within a cell microenvironment, including but not limited to a tumor, are provided.

The disclosure relates generally to alpha polyglutamated pralatrexate, formulations containing liposomes filled with alpha polyglutamated pralatrexate, methods of making the alpha polyglutamated pralatrexate and liposome containing formulations, and methods of using polyglutamated alpha polyglutamated pralatrexate and liposome containing formulations to treat hyperproliferative disorders (e.g., cancer) and disorders of the immune system (e.g., an autoimmune disease such as rheumatoid arthritis).

A theranostic composition and method for determining the cell-specific potency of drugs is provided. Further, compositions and methods useful for studying the therapeutic profile of one or more drugs within a cell microenvironment, including but not limited to a tumor, are provided.

The present invention relates to a chimeric extracellular vesicle including a stem cell derived extracellular vesicle (SC-EV) and a liposome containing a therapeutic agent. The SC-EV, derived from stem cells, provides a carrier for the delivery of therapeutic agents. The liposome encapsulates a therapeutic agent, ensuring its stability and controlled release. The combination of SC-EV and liposome allows for targeted and efficient delivery of the therapeutic and imaging agents to specific cells or tissues, enhancing its therapeutic efficacy.

Cells and lipid-based structures are described herein, in particular cells and lipid-based structures comprising membrane portions derived from cells having a label covalently attached thereto, as are compositions and kits comprising such cells and structures, and methods for using the cells and structures, in particular for determining membrane coverage and/or orientation as well as for screening methods/assays, and the like.

Dimer and monomer molecules according to general formulas (I) or (II) are useful as lipid switch molecules when incorporated into a membrane of a liposome. ##STR00001##
wherein R.sup.1 is a hydrophobic tail having at least 6 carbons and wherein R.sup.2 is selected from the group consisting of NH.sub.2, ##STR00002##
wherein, for the dimer, the linker is a saturated carbon chain having 2 to 6 carbons or is a para-xylene linker; and when R.sup.2 is charged anions are present to render the charge neutral. These molecules can bind ATP or similar small phosphorylated molecules between R.sup.2 groups, which changes the shape of the molecule or the molecules orientation within the membrane thereby acting as a switch to release a therapeutic agent from the liposome.