The present invention discloses a zonal trabecular uni-compartmental tibial plateau containing zirconium-niobium alloy on oxidation layer and preparation method, including following steps: using zirconium niobium alloy powder as raw material, conducting a 3D printing for one-piece molding to obtain an intermediate product of the uni-compartmental tibial plateau, performing hot isostatic pressing and cryogenic oxidation to obtain the uni-compartmental tibial plateau; the lower surface of the semi-tibial plateau support and the surface of the keel plate are both provided with a bone trabeculae; the zonal trabecular uni-compartmental tibial plateau adopts the structure of arranging step distributed bone trabeculaes which can reduce the fretting wear of the interface between the prosthesis and the bone, and reduce the stress shielding effect of the prosthesis on the bone tissue, homogenize the stress of the tibial plateau bone tissue, and improve the initial stability and long-term stability of the uni-compartmental tibial plateau.