The present invention relates to a method for preparing a nano diagnostic agent capable of selective staining of abnormal inflammatory tissues or tumor tissues. The method for preparing a nano diagnostic agent, according to the present invention, is characterized by comprising: a step of forming a first mixture by mixing polysorbate 80, soybean oil, and a water-insoluble staining material; a step of forming a second mixture by mixing and then heating the first mixture and a triblock copolymer; a step of forming a nano platform solid by cooling the second mixture; a step of forming a third mixture by mixing a solvent with the nano platform solid; a step of removing impurities from the third mixture; and a step of forming a nano-composite by freeze-drying the third mixture from which the impurities are removed. Thereby, the present invention can increase the search efficiency of a disease lesion by being transmitted through micropores of a tumor through direct permeation and absorption so as to precisely distinguish normal tissues from abnormal tissues.

The invention relates to a nanoparticle comprising copolymeric or homopolymeric compounds which comprise cyanoacrylate subunits according to formula 2A, wherein each R.sup.1 is independently selected from the group consisting of 2-ethylhexyl, 2-phenylethyl, neopentyl, 1-pentyl, 2-pentyl, 3-pentyl, 3,3-dimethyl-1-butyl, 3-methylbutyl, -heptyl, 2-heptyl, and 3-heptyl; and wherein the nanoparticle further comprises an active agent. The invention also provides compositions comprising the nanoparticles, and the nanoparticles for use in therapy or diagnosis, such as in the treatment of cancer or an infection, or for use in diagnostic imaging.

The present invention relates to an antibody-fluorescent dye conjugate capable of cancer cell-specific fluorescence imaging diagnosis. The fluorescent dye comprises a covalently labeled antibody and is quenched by interaction with an amino acid residue such as tryptophan, tyrosine, histidine, and methionine in the antibody and upon binding of the antibody to an antigen present on a cell surface to emit fluorescence, whereby cells having a target antigen thereon can be imaged for diagnosis. When using the antibody-fluorescent dye conjugate according to the present invention during in vitro cell assays, high-throughput screening of cells, and cytodiagnosis based on microfluidics, the presence of cancer cells having a specific antigen expressed thereon can be detected at high specificity and sensitivity without a washing process, and the position of primary and metastatic cancer cells can be detected at high contrast within a short time.

Provided are far red to near-infrared nerve-sparing fluorescent compounds, compositions comprising them, and methods of their use in medical procedures.

The instant invention provides near-infrared fluorescent biological contrast agents and methods of using them.

Nerve-specific fluorophore formulations for direct or systemic administration are described. The formulations can be used in fluorescence-guided surgery (FGS) to aid in nerve preservation during surgical interventions.

The present disclosure relates to gastrointestinal (GI) tract detection methods, devices and systems.

Disclosed is a compound of formula (I), wherein Q, U, T, A, a, b, c, and n are as defined herein. Also disclosed are methods of inhibiting ecto-5′-nucleotidase, inhibiting suppression of an antitumor immune response, inhibiting tumor growth of a cancerous tumor, inhibiting metastasis of cancer in a mammal afflicted with cancer, synergistically enhancing a response of a mammal afflicted with cancer undergoing treatment with an immunotherapeutic anti-cancer agent, potentiating an activity of an inhibitor of nicotinamide phosphoribosyltransferase in a mammal undergoing treatment of a mammal with the inhibitor, and treating preeclampsia in a mammal in need thereof, comprising administering to an animal an effective amount of a compound of formula (I).

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The present invention relates to a nanocomposite for detecting hydrogen sulfide; a method for preparing the same; a novel reactive fluorogenic compound to be used in the method; a kit for detecting hydrogen sulfide comprising the nanocomposite; and a method for providing information for the diagnosis of a disease, which causes abnormal secretion of hydrogen sulfide, by using the nanocomposite.

An electrospun nanofibrous silk fibroin mat infused with a reactive oxygen species (ROS) detection reagent is presented herein. The ROS detection agent may be Amplex red. A method of real time detection of ROS in a wound site to determine the need for a dressing change is also presented. The method includes applying an electrospun nanofibrous silk fibroin mat infused with Amplex red to a wound site of a patient and detecting a change in color of the mat. A color change indicates a higher than normal level of ROS in the wound site. Depending on the degree of color change, a therapeutic agent may be administered to the wound site and a new mat applied to measure ROS in the wound site after therapy is administered.