This invention relates to, in part, methods and compositions that are useful for the diagnosis, treatment, or prevention of a blinding eye disease, including in the discovery of drugs that are efficacious against these diseases. Diseases include, for example, age related macular degeneration and reticular pseudodrusen disease, and the methods described herein include, for example, the method named delayed near infrared analysis (DNIRA).

Activity-based probe compounds for use in labeling a cysteine protease are provided. The compounds are targeted to the protease through a specific targeting element. The compounds additionally include a detectable element, such as a fluorescent label, a radiolabel, or a chelator. In some cases, the compounds additionally include a quenching element that is released upon reaction with the protease. Also provided are compositions comprising the compounds and methods for using the compounds, for example in labeling a protease in an animal and in visualizing a tumor in an animal.

The various embodiments relate to the identification of non-viable tissue for debridement using a pH low insertion peptide conjugate. pH low insertion peptide conjugates are described having fluorescent dye for introduction to the wound area. The peptides may further be conjugated with a drug for introduction to the tissue.

One embodiment of the present invention relates to a nanoemulsion and a preparation method therefor, the nanoemulsion comprising an oil component, a surfactant, and an aqueous component, wherein the aqueous component comprises a water-soluble active ingredient, a polysaccharide, and hyaluronic acid.

A method of imaging cancer stem cells comprises disposing a population of first ultrasound-switchable fluorophorms having a first switching threshold in the biological environment, the first ultrasound-switchable fluorophores being functionalized for attachment to a first biomarker expressed by the CSCs; disposing a population of second ultrasound-switchable fluorophorms having a second switching threshold in the biological environment, the second ultrasound-switchable fluorophores being functionalized for attachment to a second biomarker expressed by the CSCs; exposing the biological environment to an ultrasound beam to form an activation region; disposing one or more of the first and/or second ultrasound-switchable fluorophores in the activation region to switch the first and/or second fluorophores from an off state to an on state; exciting the first and second ultrasound-switchable fluorophores in the activation region with a beam of electromagnetic radiation; and detecting light emitted by the first and second ultrasound-switchable fluorophores.

The present invention provides a method of diagnosing a CNS disorder comprising administering a fluorescent marker of retinal integrity to a subject and generating an image of the subject's eye, wherein the fluorescent marker is delivered by intranasal administration is also provided and fluorescent markers of retinal integrity for use in such methods. Also provided is a pharmaceutical composition comprising an annex in or a functional fragment or derivative thereof conjugated to a compound of 2 kDa or less, wherein the composition comprises annex in or a functional fragment or derivative thereof conjugated at a concentration of at least mg/ml.

The present disclosure provides polyethylene glycol-indocyanine green conjugates as well as the methods of using such conjugates, such as diagnosis, tissue imaging, temporal monitoring, and treatment.

Methods of delivering agents to structures within the eye are provided.

Methods and systems for alignment of a subject for medical imaging are disclosed, and involve providing a reference image of an anatomical region of the subject, the anatomical region comprising a target tissue, processing the reference image to generate an alignment reference image, displaying the alignment reference image concurrently with real-time video of the anatomical region, and aligning the real-time video with the alignment reference image to overlay the real-time video with the alignment reference image. Following such alignment, the subject may be imaged using, for example, fluorescence imaging, wherein the fluorescence imaging may be performed by an image acquisition assembly aligned in accordance with the alignment.

The disclosure relates to a two-dimensional (2D) bismuth nanocomposite, and a preparation method and use thereof, and belongs to the field of nanobiotechnology. The 2D bismuth nanocomposite of the disclosure is an ultra-thin bismuth nanosheet that is loaded with platinum nanoparticles and modified with indocyanine green (ICG) and surface targeting polypeptide Ang-2. The 2D bismuth nanocomposite Bi@Pt/ICG-Ang2 of the disclosure can not only realize the targeted photothermal and photodynamic combination therapy for tumors, but also realize the dual-mode imaging combining CT and fluorescence imaging.