A ferrite nano-composites with synergistic enhancement of liver specificity and preparation method and application thereof, wherein the ferrite nano-composites have both manganese ions and ethoxybenzyl group, and the molar percentage of ethoxybenzyl group to manganese ions is 25-60%. The molar percentages of manganese and ferric ions in the ferrite nanoparticles are 40-80%, and the ferrite nano-composites with manganese ions and ethoxybenzyl groups on the surface are in the particle size range of 0.2-5 nm, with preferred particle size range of 2-4 nm. With the preparation method and the application for magnetic resonance T1 imaging, the ferrite nano-composites enhance hepatocyte specificity due to the synergistic effect of manganese ions and ethoxybenzyl groups, thus achieving enhanced T1 imaging of the liver with high specificity in magnetic resonance imaging.