Embodiments of the present disclosure describe an ultra-high condition prescreening protein crystallization kit, comprising: (a) gold nanoparticles coated with PEG (polyethylene glycol); (b) ultra-high conditions scaled up to screen; (c) a plurality of cover slips with a recess; (d) a plurality of greased Linbro plates. Embodiments of the present disclosure also describe a modified hanging drop method for protein crystallization.

The disclosure relates to a microfluidic system for the screening of polymorphs, morphology, and crystallization kinetics under well-mixed, continuous-flow at controlled supersaturations. The disclosure also relates to a method for screening crystalline polymorphs and morphology, and crystallization kinetics. The microfluidic system includes a microfluidic chamber having one or more inlets, a passive mixing zone, and a trap zone. The passive mixing zone promotes mixing of solvent, solute, and optionally antisolvent under stable, controlled levels of supersaturation. The trap zone similarly has stable, controlled levels of supersaturation and correspondingly low velocity to retain solute crystals formed in the trap zone for time-dependent evaluation.

The present application provides method for operating a crystallization process model generating device. The method includes receiving selections of a solubility template, a particle size distribution template, a crystallization device template, a process time template, and a concentration factor template provided in a certain order, respectively, performing modeling of a crystallization process model by combining the selected templates, and when the crystallization process model is built, receiving an input of a condition for an injection liquid to perform a simulation for deriving a crystal suspension by inputting the injection liquid into the crystallization process model.