A system for treating wastewater comprising a coagulation-flocculation assembly having a raw wastewater inlet and a coagulated-flocculated wastewater outlet; and a slurry separator comprising an intake area configured for receiving wastewater slurry from the coagulated-flocculated wastewater outlet, a liquid outlet, a sludge outlet, and a filtration module configured to facilitate percolating of liquid therethrough and forming of a filter cake thereon. The slurry separator being configured to receive slurry at the intake area, separate the slurry to liquid and sludge by the filtration module, remove the liquid via the liquid outlet, and convey the sludge from the intake area to the sludge outlet. The system further comprises a level maintaining arrangement configured to maintain at least a minimal level of the filter cake.

A method and apparatus for collecting agricultural manure in a confined animal feeding operation includes a separator which receives heavy manure removing particulate from suspension to produce light manure. Heavy manure is collected to a volume of heavy manure sufficient to substantially fill the first tank. Within the first tank, particulate migrates, due to the influence of gravity to form a layer containing manure comprising a lesser density of particulate than is present in the volume of heavy manure. Additional heavy manure buoys the layer such that the upper surface exceeds a height of a weir. The weir is situated in a channel communicating between the first tank and a second tank configured to receive light manure from the separator.

A method and apparatus for collecting agricultural manure in a confined animal feeding operation includes a separator which receives heavy manure removing particulate from suspension to produce light manure. Heavy manure is collected to a volume of heavy manure sufficient to substantially fill the first tank. Within the first tank, particulate migrates, due to the influence of gravity to form a layer containing manure comprising a lesser density of particulate than is present in the volume of heavy manure. Additional heavy manure buoys the layer such that the upper surface exceeds a height of a weir. The weir is situated in a channel communicating between the first tank and a second tank configured to receive light manure from the separator.

Provided are: a sludge dehydrating agent which can exert a steady dehydrating effect on various types of sludge and has excellent dehydration performance; and a sludge dehydration method using the sludge dehydrating agent. The sludge dehydrating agent of the present invention with a polymer which has a constituent unit derived from a cationic monomer, wherein a value k determined from an intrinsic viscosity [η] 1 [dL/g] of a 1-mol/L aqueous sodium nitrate solution of the polymer at 30° C. and an intrinsic viscosity [η] 0.01 [dL/g] of a 0.01-mol/L aqueous sodium nitrate solution of the polymer at 30° C. in accordance with formula (I) is 0.8 to 2.2 exclusive.

k=([η]0.01−[η]1)/9   (I)

Provided are: a sludge dehydrating agent which can exert a steady dehydrating effect on various types of sludge and has excellent dehydration performance; and a sludge dehydration method using the sludge dehydrating agent. The sludge dehydrating agent of the present invention with a polymer which has a constituent unit derived from a cationic monomer, wherein a value k determined from an intrinsic viscosity [η] 1 [dL/g] of a 1-mol/L aqueous sodium nitrate solution of the polymer at 30° C. and an intrinsic viscosity [η] 0.01 [dL/g] of a 0.01-mol/L aqueous sodium nitrate solution of the polymer at 30° C. in accordance with formula (I) is 0.8 to 2.2 exclusive.

k=([η]0.01−[η]1)/9   (I)

The invention relates to compositions and methods for isolating extracellular vesicles (EVs) from a biological fluid sample. The compositions and methods of the invention are based on the combination of a polycation with an extracellular matrix forming polymer. Extracellular vesicles (EVs) are isolated from biological fluids such as blood, serum, plasma, saliva, urine or cerebrospinal fluid, or from the conditioned medium of a cell culture, such as an adult stem cell culture. The use of the isolation methods and compositions of the invention results in a higher EVs recovery, enrichment in exosomes, simplicity, cost-effectiveness, and in the isolation of EVs that retain their biological activities in vitro.

The invention relates to compositions and methods for isolating extracellular vesicles (EVs) from a biological fluid sample. The compositions and methods of the invention are based on the combination of a polycation with an extracellular matrix forming polymer. Extracellular vesicles (EVs) are isolated from biological fluids such as blood, serum, plasma, saliva, urine or cerebrospinal fluid, or from the conditioned medium of a cell culture, such as an adult stem cell culture. The use of the isolation methods and compositions of the invention results in a higher EVs recovery, enrichment in exosomes, simplicity, cost-effectiveness, and in the isolation of EVs that retain their biological activities in vitro.

Systems and methods for intensive recirculating aquaculture are provided herein. An example system includes water sourced from a first segment of a saline aquifer, a recirculating aquaculture system receiving the sourced water and producing discharge water, and a water discharge point located within a second segment of the saline aquifer disposed below the first segment of the saline aquifer.

Systems and methods for intensive recirculating aquaculture are provided herein. An example system includes water sourced from a first segment of a saline aquifer, a recirculating aquaculture system receiving the sourced water and producing discharge water, and a water discharge point located within a second segment of the saline aquifer disposed below the first segment of the saline aquifer.

A bio emulsion fuel manufacturing apparatus and method using vegetable oil is provided, including an oil tank unit configured to refine a vegetable oil introduced from an oil inlet by using a coagulant agent and a centrifugal decanter; a water tank unit configured to pretreat a water introduced from a water inlet by using a water tank catalyst; a first HHO gas infuser unit configured to introduce nano-bubbles into the water inside the water tank; a mixed oil unit connected to the oil tank unit and the water tank unit, and configured to produce a mixed oil by using an inline mixer; an ionization catalyst unit connected to the mixed oil unit and configured to convert the mixed oil to a bio emulsion fuel by using an ionization catalyst group; and a second HHO gas infuser unit configured to introduce HHO gas into the bio emulsion fuel.