This specification describes membrane based filtration and softening systems and methods. A system has a microfiltration or ultrafiltration (MF/UF) membrane unit upstream of a nanofiltration or reverse osmosis (NF/RO) membrane unit, optionally with no intermediate tank. In some cases, the system and method may be used with feed water provided at municipal line pressure to the membranes. NF/RO permeate is collected in a tank and then pumped to a header. Treated water may be drawn from the header for use or recycled to the system, for example to backwash or flush one or both of the membrane units. In a combined process, NF/RO permeate flushes the feed side of the NF/RO unit and then backwashes the MF/UF unit. In another process, the MF/UF unit and NF/RO unit are filled with NF/RO permeate before being placed in a standby mode.

This specification describes membrane based filtration and softening systems and methods. A system has a microfiltration or ultrafiltration (MF/UF) membrane unit upstream of a nanofiltration or reverse osmosis (NF/RO) membrane unit, optionally with no intermediate tank. In some cases, the system and method may be used with feed water provided at municipal line pressure to the membranes. NF/RO permeate is collected in a tank and then pumped to a header. Treated water may be drawn from the header for use or recycled to the system, for example to backwash or flush one or both of the membrane units. In a combined process, NF/RO permeate flushes the feed side of the NF/RO unit and then backwashes the MF/UF unit. In another process, the MF/UF unit and NF/RO unit are filled with NF/RO permeate before being placed in a standby mode.

This specification describes membrane based filtration and softening systems and methods. A system has a microfiltration or ultrafiltration (MF/UF) membrane unit upstream of a nanofiltration or reverse osmosis (NF/RO) membrane unit, optionally with no intermediate tank. In some cases, the system and method may be used with feed water provided at municipal line pressure to the membranes. NF/RO permeate is collected in a tank and then pumped to a header. Treated water may be drawn from the header for use or recycled to the system, for example to backwash or flush one or both of the membrane units. In a combined process, NF/RO permeate flushes the feed side of the NF/RO unit and then backwashes the MF/UF unit. In another process, the MF/UF unit and NF/RO unit are filled with NF/RO permeate before being placed in a standby mode.

A process for purifying a composition comprising water, a target substance, impurities and optionally cells, the process comprising the steps (A) and (B): (A) preparing a liquid feedstock by performing step (Ai) and/or (Aii) on the composition: (Ai) removing at least some of the cells from the composition; (Aii) concentrating the composition by removing water therefrom; and (B) passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified target substance and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v). The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

The present disclosure provides, in some embodiments, devices, methods, and kits for purifying extracellular vesicles (EVs) using size exclusion chromatography in tandem with cation exchange chromatography, which can be referred to as dual-mode chromatography (DMC).

The present invention relates to a method for purification of liquid compositions containing at least one sphingolipid and the use of a specific clay mineral for the purification of such liquid compositions.

Provided is a filtration method that includes a cleaning step and involves the use of a porous membrane, wherein the filtration method offers exceptional resistance to cleaning solutions (chemicals) (chemical liquid resistance) and exceptional filtration performance, and has a long service life. A filtration method includes the following steps: a filtration step in which a liquid to be processed that contains a material to be filtered is passed through a porous membrane configured from a resin having a three-dimensional mesh structure, and a filtrate is separated from the material to be filtered; and a cleaning step in which the porous membrane has a cleaning solution passed therethrough or is immersed in the cleaning solution so that the interior of the porous membrane is cleaned.

A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

A system for filling multiple sterile containers includes a filter with an inlet port and multiple outlet ports, the outlet ports being pre-attached to sterile containers by respective filling lines of each container. Each container has an interior and each of the respective filling lines are connected to a respective container interior. The respective filling lines are sealed to the outlet ports and the containers such that the container interiors are isolated from an external environment except the inlet port, via the filter, forming a combined interior volume which is sterile. A container that is connectable to an outlet port the system has a bladder, a first tube and a second tube connected to the bladder, and a sterilizing filter. The container, the first tube and the second tube, and the sterilizing filter are sterile before water is flowed through the sterilizing filter into the bladder.