In one embodiment, an optical spectroscopy probe includes an optical fiber having a distal tip and a microfluidic filtering chamber attached to the distal tip of the optical fiber, the chamber comprising a microfluidic membrane adapted to enable liquid to enter the chamber but prevent particles from entering the chamber.

A microfluidic tissue dissociation and filtration device simultaneously filters large tissue fragments and dissociates smaller aggregates into single cells, thereby improving single cell yield and purity. The device includes an inlet coupled to a first microfluidic channel at an upstream location and a first outlet at a downstream location. A first filter membrane is interposed between the first microfluidic channel and a second microfluidic channel, wherein the second microfluidic channel is in fluidic communication with the first microfluidic channel via the first filter membrane. The first filter membrane operates under a tangential flow format. A second outlet is coupled to a downstream location of the second microfluidic channel and includes a second filter membrane interposed between the second outlet and the second microfluidic channel. The dual membrane device increased single cell numbers by at least 3-fold for different tissue types.

A lateral filter array microfluidic (LFAM) device for highly efficient immunoaffinity isolation of target cells from a population of cells. The LFAM device may include of one or more serpentine main channels incorporated with lateral filter arrays. Antibodies are immobilized on the channel surface including the lateral filters and are capable of specific binding to one or more biomolecules on the surface of the target cell. The device may include one or more arrays of lateral filters with different sizes. The overall filters sizes are close to the diameter of the target cell, therefore the interaction between biomarkers on the target cells and corresponding antibodies immobilized on the filter surface is largely strengthened due to the direct contact between target cells and lateral filters. Methods include flowing a population of cells through an antibody-coated LFAM device for target cells capture, followed by washing the device to remove non-specific captured cells.

Disclosed herein are rolled-membrane microfluidic diffusion devices and corresponding methods of manufacture. Also disclosed herein are three-dimensionally printed microfluidic devices and corresponding methods of manufacture. Optionally, the disclosed microfluidic devices can function as artificial lung devices.

Disclosed herein are rolled-membrane microfluidic diffusion devices and corresponding methods of manufacture. Also disclosed herein are three-dimensionally printed microfluidic devices and corresponding methods of manufacture. Optionally, the disclosed microfluidic devices can function as artificial lung devices.

Fluorinated metal nanoparticles (f-MNPs) are metal nanoparticles and/or semiconductor nanoparticles having a plurality of fluorinated alkyl ligands attached to the surfaces of the nanoparticles where the f-MNPs are greater than 20 nm in cross-section. The f-MNPs can be used as a surfactant to form active f-MNPs stabilized aqueous droplet, which can be used in a light-mediated microfluidic device where droplet movement, merging, splitting, and sorting can be carried out as directed by one or more laser beams focused onto one or more spots of light-mediated microfluidic device.

An extraction chamber includes a main channel, a plasma channel, and a plurality of side channels fluidly connecting the main channel to the plasma channel. Each of the plurality of side channels has an average diameter of at most 200 m at a narrowest cross section, and the extraction chamber has a separation efficiency of at least 95%. A multilevel extraction chamber, includes a main channel, a secondary channel fluidly connected to the main channel, a plasma channel fluidly connected to the secondary channel, a first set of a plurality of side channels fluidly connecting the main channel to the secondary channel, and a second set of a plurality of side channels fluidly connecting the secondary channel to the plasma channel. Each of the first set of side channels has an average diameter that is greater than the average diameter of each of the second set of side channels, and the extraction chamber has a separation efficiency of at least 95%.

There are provided a liquid feeding assistance device, a cell processing apparatus, and a liquid feeding assistance method that can suppress the pulsation of liquid flowing in a flow passage.

A liquid feeding assistance device includes a tube that is expandable and contractible depending on a flow velocity of liquid flowing in the tube, and a gripping member that grips the tube such that an orthogonal cross-sectional area of a flow passage formed by the tube, which is an area of a cross section of the flow passage orthogonal to a flow direction of the liquid, is smaller than an orthogonal cross-sectional area of the flow passage in a natural state. The tube is a single-use tube.

A biofluid card assembly for isolating extracellular vesicles present in biofluids includes a biofluid card having a plurality of layers; and a fastener, the biofluid card includes a top lid with at least one opening configured to receive at least one biofluid sample including principal components and extracellular vesicles present therein, a biofluid separation membrane assembly disposed adjacent the top lid and configured to separate and direct the extracellular vesicles, at least one extracellular vesicle capture membrane having at least one extracellular vesicle capture agent formed thereon or embedded therein, each disposed below and adjacent the biofluid separation membrane assembly and configured to receive the extracellular vesicles, wherein each of the at least one extracellular vesicle capture agent includes a binding agent configured to bind to the extracellular vesicle having predetermined binding sites, and a bottom lid disposed adjacent the at least one extracellular vesicle capture membrane.

An extraction chamber includes a main channel, a plasma channel, and a plurality of side channels fluidly connecting the main channel to the plasma channel. Each of the plurality of side channels has an average diameter of at most 200 m at a narrowest cross section, and the extraction chamber has a separation efficiency of at least 95%. A multilevel extraction chamber, includes a main channel, a secondary channel fluidly connected to the main channel, a plasma channel fluidly connected to the secondary channel, a first set of a plurality of side channels fluidly connecting the main channel to the secondary channel, and a second set of a plurality of side channels fluidly connecting the secondary channel to the plasma channel. Each of the first set of side channels has an average diameter that is greater than the average diameter of each of the second set of side channels, and the extraction chamber has a separation efficiency of at least 95%.