The present disclosure relates to a process for manufacturing a sterilizing filter comprising a plurality of hollow fiber membranes having a large inner diameter.

The present invention aims to provide a porous separation membrane that does not suffer a significant decrease in the protein permeability even after long term use. The porous separation membrane has an asymmetric structure with a dense layer forming one surface layer and with a coarse layer forming the other surface layer, supports a biocompatible polymer, and meet the requirements (1) and (2) given below in surface analysis of a cross section containing the dense layer and the coarse layer performed by TOF-SIMS: (1) the minimum value of normalized intensity of the ion signal attributed to the biocompatible polymer in the coarse layer is 0.15 times or more of the maximum value, and (2) the normalized average intensity of the ion signal attributed to the biocompatible polymer in the dense layer is 2.0 times or more of the normalized average intensity of the ion signal attributed to carboxylic acid in the coarse layer.

The present invention aims to provide a porous separation membrane that does not suffer a significant decrease in the protein permeability even after long term use. The porous separation membrane has an asymmetric structure with a dense layer forming one surface layer and with a coarse layer forming the other surface layer, supports a biocompatible polymer, and meet the requirements (1) and (2) given below in surface analysis of a cross section containing the dense layer and the coarse layer performed by TOF-SIMS: (1) the minimum value of normalized intensity of the ion signal attributed to the biocompatible polymer in the coarse layer is 0.15 times or more of the maximum value, and (2) the normalized average intensity of the ion signal attributed to the biocompatible polymer in the dense layer is 2.0 times or more of the normalized average intensity of the ion signal attributed to carboxylic acid in the coarse layer.

An example porous composite membrane for solvent extraction is provided. The porous composite membrane includes a Janus membrane with a first side and a second side opposing the first side. The first side exhibits hydrophobic characteristics and the second side exhibits hydrophilic characteristics. At least one of the first side or the second side is sized to perform nondispersive membrane solvent extraction.

An example porous composite membrane for solvent extraction is provided. The porous composite membrane includes a Janus membrane with a first side and a second side opposing the first side. The first side exhibits hydrophobic characteristics and the second side exhibits hydrophilic characteristics. At least one of the first side or the second side is sized to perform nondispersive membrane solvent extraction.

A method for reducing a contaminating DNA content of a preparation containing AAV capsids and contaminating DNA, comprising the steps of a) Performing an extraction of DNA with a solid phase bearing positive charges at its surface said solid phase is contacted with the preparation at a pH of 7.0±1.0, and a salt concentration of 10 mM to 200 mM yielding a first fraction, (b) Diafiltering the first fraction by a first tangential flow filtration to obtain a second fraction, (c) Treating the second fraction with DNase, (d) Diafiltering the DNase treated second fraction obtained by step c) by a second tangential flow, (e) filtration to a buffer with pH of 7.0±1.0, and a salt concentration of 10 mM to 20 mM to yield a third fraction, and optionally (f) Concentrating the third fraction by tangential flow filtration before supplemental chromatography.

A method for reducing a contaminating DNA content of a preparation containing AAV capsids and contaminating DNA, comprising the steps of a) Performing an extraction of DNA with a solid phase bearing positive charges at its surface said solid phase is contacted with the preparation at a pH of 7.0±1.0, and a salt concentration of 10 mM to 200 mM yielding a first fraction, (b) Diafiltering the first fraction by a first tangential flow filtration to obtain a second fraction, (c) Treating the second fraction with DNase, (d) Diafiltering the DNase treated second fraction obtained by step c) by a second tangential flow, (e) filtration to a buffer with pH of 7.0±1.0, and a salt concentration of 10 mM to 20 mM to yield a third fraction, and optionally (f) Concentrating the third fraction by tangential flow filtration before supplemental chromatography.

The present invention relates to systems and methods for catalytic removal of oxidized contaminants (for example, nitrite, chromate, chlorate, trichloroethene, CFC-11, 4-nonylphenol, RDX, and perfluorooctanoate) from water and wastewater. In some aspects, the catalytic method of removing oxidized contaminants comprises using precious metal nanoparticles as catalysts to reduce the oxidized contaminants.

A cartridge is provided for dialysis or other blood processing therapy. In the cartridge, fibers may be substantially uniformly distributed near a midplane, but near an end, in the inter fiber space, there may be void flow channels, which may cause fluid flow in the inter fiber space to transition within a short region to uniform flow with minimal stagnation zones. Void flow channels may be be radially oriented, introducing fluid from the outer circumference, or axially oriented, introducing fluid along the axial direction through passageways through the potting material. The fluid flow in the inter fiber space may be perpendicular to the fibers, or radial with respect to a cartridge longitudinal axis. The cartridge may have blood flow in the inter fiber space, and flow of dialysate or ultrafiltrate in the lumens of the fibers, or the opposite situation.

A cartridge is provided for dialysis or other blood processing therapy. In the cartridge, fibers may be substantially uniformly distributed near a midplane, but near an end, in the inter fiber space, there may be void flow channels, which may cause fluid flow in the inter fiber space to transition within a short region to uniform flow with minimal stagnation zones. Void flow channels may be be radially oriented, introducing fluid from the outer circumference, or axially oriented, introducing fluid along the axial direction through passageways through the potting material. The fluid flow in the inter fiber space may be perpendicular to the fibers, or radial with respect to a cartridge longitudinal axis. The cartridge may have blood flow in the inter fiber space, and flow of dialysate or ultrafiltrate in the lumens of the fibers, or the opposite situation.