Disclosed is a continuous process for the purification of steviol glycosides such as Rebaudioside D and/or Rebaudioside M extracted from the dried stevia leaves or extracted from a fermentation broth using continuous simulated moving bed processes and nanofiltration without the addition of organic solvents to obtain a purified steviol product comprising sweet steviol glycosides. The sweet steviol glycosides can be used as substitutes for caloric sweeteners in beverages and in other food items.

The present invention relates method for purifying antibodies, said method comprising a limited number of steps while still allowing obtaining high yields of purified antibodies with an appropriate degree of purity. Briefly, this method comprises only filtration and precipitation steps, omitting the need for chromatography steps.

Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.

A system for treating a tetraalkylammonium hydroxide-containing liquid having a high-pressure type reverse osmosis membrane device concentrating a liquid to be treated containing tetraalkylammonium hydroxide at a concentration side, and a line for supplying the concentrated liquid to be treated by the reverse osmosis membrane device to an evaporator further concentrating the concentrated liquid to be treated.

A system for treating a tetraalkylammonium hydroxide-containing liquid having a high-pressure type reverse osmosis membrane device concentrating a liquid to be treated containing tetraalkylammonium hydroxide at a concentration side, and a line for supplying the concentrated liquid to be treated by the reverse osmosis membrane device to an evaporator further concentrating the concentrated liquid to be treated.

The present invention relates to a method for concentrating, with low energy, a solute-containing solution in a state of multiple-no osmotic pressure difference (M(multiple)−Δπ=0 RO), and, more specifically, to a method for concentrating, with low energy, an solute-containing solution intended to be concentrated, as a low pressure in a state of multiple-no osmotic pressure difference. The method for concentrating a solution containing a solute at a low pressure in a state of multiple-no osmotic pressure difference, of the present invention, consumes less energy, enables concentration to be performed until a saturated aqueous solution with a maximum solute concentration is obtained or the concentration of the solute becomes 100% even though an extraction solvent is not used, and does not require the use of an additional osmosis-inducing solution.

Separation of a gas mixture comprising first and second gases may be improved using two cascaded stages of gas separation membrane modules that includes the additional techniques of cooling the feed gas stream that is fed to the feed stage and using a portion of the feed stage retentate as a sweep gas on the feed stage.

This application relates generally to the processing of egg protein isolates for use in various food, sports nutrition and nutraceutical applications, methods of making egg protein isolates, and apparatus for making egg protein isolates. More particularly, the application relates to a method of deflavoring egg products and concentrating the protein content to. The method can include providing liquid egg; deashing the liquid egg; concentrating the liquid egg; and desugaring the liquid egg, wherein deashing is accomplished at a pressure of less than 100 psi; and optionally the volume of water used is 0.5 to 7 diafiltration volumes.

A multiple-loop tangential flow filtration apparatus for concentrating fluids is described herein. The apparatus comprises a plurality of tube loops for receiving fluid therethrough, each tube loop comprising a respective filter, and a common feed pump for driving the fluid across each respective filter. The plurality of tube loops are coupled to the common pump via a common feed line.

A multiple-loop tangential flow filtration apparatus for concentrating fluids is described herein. The apparatus comprises a plurality of tube loops for receiving fluid therethrough, each tube loop comprising a respective filter, and a common feed pump for driving the fluid across each respective filter. The plurality of tube loops are coupled to the common pump via a common feed line.