An automated modular filtration system, particularly for low volume tangential flow filtration processes, comprises a plurality of filtration modules formed as separate assemblies and at least one control unit for jointly controlling filtration processes of individual filtration units. Each filtration module contains at least one individual filtration unit for executing a filtration process independent of the other filtration units, first input ports for receiving a first type of fluids, second input ports for receiving a second type of fluids, and exit ports for outputting unused system fluids. First type fluids are process fluids are specific to the filtration processes executed in individual filtration units. Second type fluids are system fluids not specific to filtration processes executed in the individual filtration units. The second input and exit ports establish inter-module connections so system fluids can be forwarded from one filtration module to an adjacent filtration module of the filtration system.

There is provided a single pass electro-separation system for separating substantially all of a charged molecule from a fluid stream in a single pass, the system comprising an assembly adapted to be disposed between a cathode and an anode. The assembly comprises: at least a first, second, and third separation membrane each having defined pore sizes; a first spacer disposed between the first and second separation membranes and having a void extending from an inlet to an outlet to define a first fluid flow path for a first fluid stream between the first and second separation membranes; and a second spacer disposed between the second and third separation membranes and having a void extending from an inlet and an outlet to define a second fluid flow path for a second fluid stream between the second and third separation membranes

A filter module (1) has a housing (2) formed from a plastic and a filter (3) made from a plastic is arranged in the housing (2). The housing (2) is bonded adhesively to the filter (3) by an adhesive (15) via a first bonding surface (7, 18) made from a first plastic and via a second bonding surface (14, 19, 21) made from a second plastic. The adhesive bonding of the first bonding surface (7, 18) and the second bonding surface (14, 19, 21) is accomplished via an intermediate piece (10, 20) with the bonding surfaces (14, 18, 21) made from a plastic that is identical to or different from the first plastic. At least one of the bonding surfaces (7, 18, 19, 14, 21) has been activated by plasma or corona pre-treatment prior to the adhesive bonding.

A molecular filtration device and method of use capable of filtering and purifying molecules of a particular characteristic, wherein the amount of molecule to be filtered may be in the nanogram range and may be dispersed in a relatively large volume of solution. The resultant elution may include a relatively high concentration of desired molecule, due to a relatively small volume.

Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.

A single-use fluid storage and filtration system includes a process vessel, a filter module including a hollow fiber filter element, and a drive module coupled to the filter module. The filter module is fixed to the process vessel and is in fluid communication with the process vessel for filtering a fluid received from the process vessel. The drive module includes a pump to induce flow of the fluid between the filter module and the process vessel. The process vessel, filter module and drive module comprise a single integrated and sterilized assembly.

A fluid filtration assembly includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. The filter cartridge can be a hollow fiber filter. The plunger pump can be configured to induce alternating tangential flow in at least a portion a the assembly. The fluid filtration assembly can be provided as a disposable single-use arrangement.

A fluid filtration assembly includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps is a diaphragm pump or a plunger pump. The fluid storage vessel can be a bioreactor.

In various embodiments, the present invention provides a process for separating target proteins from non-target proteins in a sample comprising increasing the concentration of the target proteins and non-target proteins in the sample and subsequently delivering the concentrated sample to a chromatography device. In other embodiments, the invention relates to a process for increasing the capacity of a chromatography device for a target protein by delivering a concentrated sample comprising the target protein to a chromatography device.

The present invention provides a disposable ultra-filtration system comprising a disposable pipetting tip and a disposable ultra-filtration cartridge, wherein the cartridge includes a membrane filtration chamber and a dead-end channel. In use, a piston in the pipette pressurizes air within the channel; the pressurized air can subsequently move the piston and cause a reverse flow back through the membrane of the cartridge, unplugging the pores thereof. Also disclosed is an automated workstation incorporating the disposable ultra-filtration system, and a system comprising the automated workstation, useful for measuring the free therapeutic drug concentration and free hormone concentration in a sample.