A method of operating a high velocity cross flow dynamic membrane filtration includes feeding a fluid stream into a pressure vessel, in which the vessel defines a treatment chamber containing a disc membrane assembly having a first support shaft and a second support shaft, each support shaft defining a longitudinal axis about which is positioned a plurality of axially spaced membrane discs. The method further includes distributing the fluid stream over at least a portion of the disc membrane assembly. The method also includes discharging a first portion of the fluid stream from the vessel and discharging a second portion of the fluid stream from the vessel. The method additionally includes rotating the first support shaft and the second support shaft in a first direction. The rotating includes modulating a rotation rate in response to the flow rate of the second portion of the fluid stream.

A means capable of simply and efficiently concentrating a cell suspension. A concentrator has a culture vessel having a first port and a second port, a server bag having a port, a case having a hollow fiber bundle in the internal space, a filtering device having an inlet port, a first outlet port, and a second outlet port, a collection vessel having a port, a liquid supply circuit connected to the first port, the inlet port, and the server bag's port so that flow passages are switchable, a liquid discharge circuit connected to the second port, the first outlet port, the second outlet port, and the collection vessel's port so that flow passages are switchable, a liquid supply mechanism having a switching mechanism, a supply pump, and a discharge pump, and a rotation mechanism rotating the filtering device.

The reverse osmosis centrifuge converts rotational energy into fluid velocity and conserves the energy placed into the concentrate. As concentrate travels back towards the center of the reverse osmosis centrifuge, the velocity of the fluid is converted into rotational force, thus conserving energy. To accomplish this, the reverse osmosis centrifuge includes a stationary cylindrical housing having a vacuum chamber and a vacuum pump for generating vacuum pressure in the vacuum chamber, a driveshaft coupled to a membrane cylinder rotatable within the stationary cylindrical housing, the membrane cylinder having a plurality of vertical desalination membranes, and an energy recovery turbine. The reverse osmosis centrifuge can be placed on the concentrate or waste stream outlet of a desalination or reverse osmosis facility to increase freshwater production. Through using the methods described above, plant water production can be increased up to 40%, which in turn has a dramatic effect on plant profitability.

A high velocity cross flow dynamic membrane filtration system includes a disc membrane assembly having a frame and at least two support shafts. Each support shaft defines a longitudinal axis about which is positioned a plurality of axially spaced membrane discs, with each shaft further coupled to the frame. A permeate tube is coupled to each support shaft and in fluid communication with the membrane discs associated with that support shaft. A vessel defines a treatment chamber and is configured to removably support the disc membrane assembly within the treatment chamber. The vessel further includes a wall. The filtration system also includes a drive system. The permeate tubes are configured to extend through a portion of the vessel wall when the disc membrane assembly is positioned within the treatment chamber. The permeate tubes are further configured for rotation by the drive system.

A filtration cell (10) for a biological sample having an outer housing (12) defining a first chamber and an inner housing (14) defining a second chamber is disclosed. The inner housing is disposed within the first chamber and rotatable with respect to the outer housing and at least a portion of the inner housing includes a filtration membrane (52). Upon rotation of the inner housing, a first portion of the biological sample passes from the first chamber into the second chamber and a second portion of the biological sample is restrained in the first chamber. Alternatively, the filtration cell may also include a rotation element disposed in the inner housing. Upon rotation of the rotation element with respect to the inner housing, a first portion of the biological sample to passes from the second chamber into the first chamber and a second portion of the biological sample is restrained in the second chamber.

A membrane separation device is disclosed along with systems and methods employing the device in blood processing procedures. In one embodiment, a spinning membrane separator is provided in which at least two zones or regions are created in the gap between the membrane and the shell, such that mixing of the fluid between the two regions is inhibited by a radial rib associated with the membrane that decreases the gap between the membrane and the shell to define two fluid regions, the ridge isolating the fluid in the two regions to minimize mixing between the two. Automated systems and methods are disclosed for separating a unit of previously collected whole blood into components, such as concentrated red cells and plasma, for collecting red cells and plasma directly from a donor in a single pass, and for cell washing. Data management systems and methods and priming methods are also disclosed.

Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.

Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.

A network of cell processing systems including a plurality of cell processing instruments and a server computer. Each cell processing instrument includes a control circuit configured to operate the cell processing instrument according to a modifiable process parameter, a component of the cell processing instrument and a sensor configured to measure a characteristic of the component. The server computer is disposed remotely from the cell processing instruments, and is configured to transmit a request message for a value measured by the sensor, receive a response message based on the request message, and generate a notification message based on the response message.

The present invention provides a rare cell isolation device including: a first body which is disposed above a filtration membrane and includes a first inlet for injecting a biospecimen; and a second body which is disposed under the first body and bonded to the filtration membrane, wherein the first body and the second body have a disk-shaped structure to be rotatable around their centers, and the filtration membrane is disposed to be separated from the center of the second body in a radial direction.