Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 μm or smaller, and 0<B/A≤0.5 is satisfied in a case where an average hole diameter of the first filter membrane is A and an average hole diameter of the second filter membrane is B; or an average hole diameter of the first filter membrane is 20 μm or smaller, and the second filter membrane is an ultrafiltration membrane.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

A filter device includes one or more filter membranes, and a filter housing enclosing the one or more filter membranes. Each of the filter membranes includes a base membrane made of a ceramic material, and a plurality of through holes. The base membrane is coated with a coating material.

A centrifugal filtration device is provided. The centrifugal filtration device has filtration membrane that filtrates liquid; cartridge that supports filtration membrane and that forms liquid chamber together with filtration membrane, wherein liquid chamber holds the liquid therein; and rotating member that rotates around rotation center and that supports cartridge such that filtration membrane is positioned outward of liquid chamber with respect to rotation center. Rotating member has a path that is connected to liquid chamber, and at least a part of a liquid contact part of the path that is in contact with the liquid is formed of titanium or a titanium alloy.

A method of making a polymer membrane, the method including providing a first monomer solution having a first solvent, a second monomer solution having a second solvent, and a substrate having a surface, and including electrospraying the first monomer solution onto the substrate surface and electrospraying the second monomer solution onto the substrate surface to form the polymer membrane on at least a portion of the substrate surface.

Provided herein are graphene materials, fabrication processes, and devices with improved performance and a high throughput. In some embodiments, the present disclosure provides graphene oxide (GO) materials and methods for forming GO materials. Such methods for forming GO materials avoid the shortcomings of current forming methods, to facilitate facile, high-throughput production of GO materials.

Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 .Math.m or smaller, and 0 < B/A ≤ 0.5 is satisfied in a case where an average hole diameter of the first filter membrane is A and an average hole diameter of the second filter membrane is B; or an average hole diameter of the first filter membrane is 20 .Math.m or smaller, and the second filter membrane is an ultrafiltration membrane.

A method of vacuum membrane filtration including placing a membrane filter between a filtration base and a pouring funnel, an upper side of the filtration base having a membrane bearing area with a bearing structure and a supporting contour surrounding the bearing structure and the supporting contour having at least one notch in flow connection with a bottom side of the membrane bearing area, detachably mounting the pouring funnel on the filtration base thereby clamping the membrane filter between the filtration base and the pouring funnel, applying suction to the filtration base such that the membrane filter is pulled against the bearing structure and comes into contact with the supporting contour, and dismounting the pouring funnel from the filtration base while still applying the suction, causing an outer rim of the membrane filter to bulge upward from the supporting contour and uncover the at least one notch.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.