A method of recovering draw agent utilised in a forward osmosis membrane cell, the method comprising the steps of passing diluted draw agent to a vapour-liquid separator; using the vapour-liquid separator to separate draw agent vapour and solvent; and condensing draw agent vapour.

The present disclosure provides, in part, a receptacle for filtering a liquid. The receptacle comprises a plurality of hollow fibers extending the length of the receptacle and at least one solid absorbent material occupying a space between the plurality of hollow fibers. Each hollow fiber comprises at least one opening and a lumen defined by the walls thereof, allowing the liquid to flow through. The hollow fiber walls have a porosity profile selective to passage of waste materials contained in the liquid from the lumen to the solid absorbent material(s), thereby filtering the liquid. Also provided is a system as well as a method for filtering and recycling a cell culture medium.

Systems and processes for purifying and concentrating a liquid feed stream are disclosed. In the systems, the concentrated liquid output from the high pressure side of a reverse osmosis stage is used as the draw solution in the low pressure side of the reverse osmosis stage in a configuration called osmotically assisted reverse osmosis. This reduces the osmotic pressure differential across the membrane, permitting high solute concentrations to be obtained, hastening the purification of the liquid. Reduced system pressures are also obtained by arranging multiple osmotically assisted reverse osmosis stages in a cross-current arrangement. Overall system energy consumption is reduced compared to conventional thermal processes for high concentration streams.

The present disclosure relates to a new use of a tangential flow filtration device and a continuous concentration system for producing an ultra-high concentration medical exosome solution.

A separation system includes first and second separation parts each having a separation membrane and provided with a fluid supply port, a permeate fluid exhaust port, and a non-permeate fluid exhaust port, an intermediate connecting part for connecting the permeate fluid exhaust port of the first separation part and the fluid supply port of the second separation part, a supply pipe connected to the fluid supply port of the first separation part, in which a mixed fluid flows at a pressure higher than an atmospheric pressure, and a pressure reducing part connected to the permeate fluid exhaust port of the second separation part, for reducing a pressure inside the permeate fluid exhaust port to a pressure lower than the atmospheric pressure. A pressure inside the intermediate connecting part is lower than a pressure inside the supply pipe and not lower than the atmospheric pressure.

The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.

A method exosome purification and characterization is contemplated, comprising the steps of secondary two-stage tangential ultrafiltration to produce an extracted solution, pretreatment of the extracted solution for characterization, characterization of the extracted solution to detect particle size and concentration, and freeze-drying of the extracted solution. An exosome purification integrated device is also contemplated. Through the disclosed methods and devices, exosomes may be better purified and characterized in a manner that results in high practical value to overcome the problems associated with conventional exosome purification processes on the market today, including tedious purification processes, long durations, and high costs.

To provide biotechnological filtration possibilities with which the danger of contamination is low and at the same time an optimum flow profile can be achieved, the use of a filter module 100 is proposed, the latter having, in a filter housing 110, a filter, with a bundle of hollow fibres 114, and a centrifugal pump rotor 132, for a biotechnological production method, and a filter module 100 for the filtration of a biotechnological liquid L, having a filter housing 110, a filter arranged in the filter housing 110 with a bundle of hollow fibres 114, a centrifugal pump rotor 132 arranged in the filter housing 110 such that it is fluidically connected to the interior of the hollow fibres 114, wherein the centrifugal pump rotor 132 is able to be magnetically driven such that it can force a liquid L through the hollow fibres 114, a first port 102 for the supply of a biotechnological liquid L to be filtered, which first port 102 is fluidically connected to the interior of the hollow fibres 114 of the filter, a second port 104 for the removal of a biotechnological liquid L to be filtered, which second port 104 is fluidically connected to the interior of the hollow fibres 114 of the filter, and a third port 107 for the removal of precipitated waste liquid, which third port 107 is fluidically connected to the exterior of the hollow fibres 114, and a filtration device 1100, 1200, 1300 having a filter module 100, which is able to be coupled to a drive unit, and a drive unit for magnetically driving the centrifugal pump rotor 132 of the filter module 100, which drive unit can generate dynamic magnetic fields for magnetically driving and magnetically supporting the centrifugal pump rotor 132 when the filter module 100 is coupled to the drive module.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.