Embodiments of the present application relate to batches of bupivacaine multivesicular liposomes (MVLs) prepared by a commercial manufacturing process using independently operating dual tangential flow filtration modules.

Embodiments of the present application relate to batches of bupivacaine multivesicular liposomes (MVLs) prepared by a commercial manufacturing process using independently operating dual tangential flow filtration modules.

A plant cell co-expressing at least one casein protein and at least one kinase. The at least one casein protein is phosphorylated by the at least one kinase in vivo. Casein micelles comprising phosphorylated κ-casein and at least one of αS1-casein, αS2-casein, and β-casein can be made in vivo and/or in vitro. The casein micelles can be used to make food products including milk and cheese.

Provided is a novel Nanofiltration-DiaNanofiltration (NF-DiaNF) system and method for extracting divalent ions from saline water (e.g., seawater) to produce solutions rich in divalent ions (in particular Mg2+, Ca2+ and SO42−), while minimizing the concentrations of undesirable species (e.g., Cl−, Br−, B and Na+). The solutions may be added to water (e.g., desalinated, soft, drinking or irrigation water) to enrich the water with divalent ions, thereby improving its quality.

This specification relates to preparing a purified human milk oligosaccharide (HMO) from an HMO solution by a process comprising nanofiltration, as well as processes for making foods, dietary supplements, medicines and infant formulas comprising a purified HMO. This specification also relates to purified HMOs and foods, dietary supplements, medicines and infant formulas prepared by processes disclosed in this specification.

Disclosed herein is a single pass cross flow diafiltration system comprising: a filtration module having; two or more filtration segments fluidly connected in series, each having an upstream side and a downstream side; wherein each filtration segment comprises hollow fiber filter membranes, and wherein each filtration segment has a selected length; wherein the hollow fiber filter membranes of each filtration segment have a selected inner diameter; wherein the selected inner diameter of each filtration segment may be the same or different, provided that at least one selected inner diameter differs from another selected inner diameter, and provided that the two or more filtration segments are arranged such that no selected inner diameter in a given filtration segment is larger on the upstream side; one or more pumps, mounted to urge fluid flow; and one or more points of introduction of a diadiluent, each of said points of introduction being fluidly connected to an upstream filtration segment.

The invention relates to a method for treating sugar comprising: placing a coloured sugar juice in contact with an ion exchange resin so as to charge the resin with colouring agents and to collect a bleached sugar juice; regenerating the colouring-charged resin, comprising: placing the charged resin in contact with a regeneration brine comprising a chloride salt; and collecting a regeneration effluent, the regeneration effluent comprising at least three fractions A, B and C, fraction A having a higher concentration of chloride salt than fractions B and C; and recycling the regeneration effluent, comprising: nanofiltration of fraction A of the regeneration effluent in order to obtain a first permeate and a first retentate; diafiltration of the first retentate, said diafiltration comprising: dilution of the first retentate with the fraction B of the regeneration effluent; nanofiltration of the mixture in order to obtain a second permeate and a second retentate; mixing of the first permeate with the second permeate and fraction C of the regeneration effluent,

Method for obtaining low molecular weight heparins (LMWH) with a weight average molecular weight distribution between 3.0 and 5.0 kDa comprising at least one concentration step by tangential flow filtration (TFF). The method is particularly useful for the preparation of bemiparin and enoxaparin without the use of fractional precipitation nor the use of alcoholic solutions. In particular, the preparation of LMWH is obtained by depolymerization of heparin and filtration (TFF ultrafiltration and/or diafiltration) of the depolymerized heparin without the use of fractional precipitation and without an alcoholic solution.

The present application provides methods and systems for viral clearance for purifying an antibody from a sample comprising one or more impurities including viral particles. The method is conducted in a system which includes a hydrophobic interaction chromatography (HIC) column and a virus retentive filtration (VRF) system. The HIC column and the VRF system are connected inline in a continuous processing system, and the VRF system comprises at least two filter trains in parallel.

Embodiments of the present application relate to commercial manufacturing processes for making bupivacaine multivesicular liposomes (MVLs) using independently operating dual tangential flow filtration modules.