The present disclosure relates to methods and systems for the continuous production of recombinant proteins. In particular embodiments, the disclosure relates to methods and systems using capture chromatography, post-capture chromatography, virus filtration, and ultrafiltration/diafiltration for the continuous production of recombinant proteins.

The present invention relates to modification to permeate channels and permeate materials in a cross-flow filtration system to improve performance in counter current filtration having both retentate channels and permeate channels wherein a solution is pumped through one of the channels and drawn through a membrane to one of the other channels to assist in positive pressure driven filtration by using the osmotic pressure, concentration, or preferential solubility difference between the retentate and permeate flow streams thereby increasing or altering the flux through the membrane separating the flow streams.

A method of increasing height in a pediatric subject comprises enterally administering an exosome-enriched product comprising intact bovine milk-derived exosomes to the pediatric subject in need thereof. A method of promoting linear bone growth in a pediatric subject comprises enterally administering an exosome-enriched product comprising intact bovine milk-derived exosomes to the pediatric subject in need thereof. A method of obtaining an exosome-enriched product from cheese whey comprises subjecting the cheese whey to microfiltration (MF), ultrafiltration (UF), and diafiltration (DF) steps, wherein the MF, UF, and DF steps employ, successively, membranes with cut off values which gradually decrease in size with each filtration step, wherein the cheese whey is sweet cheese whey and has a pH from about 6.0 to about 6.5.

The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure.

A method and apparatus for conducting chemical reactions under controlled conditions, where one compound is in stochiometric excess over the other, using membrane filtration.

A method for separation of proteins, particularly plant proteins, from salts and phenolic compounds in a liquid comprising said proteins dissolved in said liquid is provided. The method includes a step of subjecting said liquid to a first cross-flow membrane filtration process wherein the first salts and at least a portion of the phenolic compounds migrate across the membrane into a first permeate and the proteins are retained in a first retentate; followed by a step of adding water, or one or more second salts and water to the first retentate, while continuing the membrane filtration process, to create a diafiltrate containing at least a portion of said phenolic compounds and the added second salts.

Provided is a novel Nanofiltration-DiaNanofiltration (NF-DiaNF) system and method for extracting divalent ions from saline water (e.g., seawater) to produce solutions rich in divalent ions (in particular Mg2+, Ca2+ and SO42), while minimizing the concentrations of undesirable species (e.g., Cl, Br, B and Na+). The solutions may be added to water (e.g., desalinated, soft, drinking or irrigation water) to enrich the water with divalent ions, thereby improving its quality.

In accordance with the invention, provided herein are methods for purifying recombinant adeno-associated (rAAV) vector particles.

A multiple tangential flow filtration (TFF) apparatus includes a plurality of tangential flow filtration (TFF) systems, a support frame defining a plurality of stations for supporting individual TFF systems and a single controller for controlling the plurality of tangential flow filtration systems. Each of the systems include at least a product vessel, a tangential flow filtration (TFF) filter and a pump for circulating a flow of liquid between said product vessel and said filter.

A technology that provides various modular biomimetic microfluidic modules emulating varieties of microvasculature in body. These microfluidic-base capillaries and lymphatic Technology modules are constructed as multilayered-microfluidic microchannels of various shapes, and aspect ratios using diverse biocompatible microfluidic polymers. Then, various semipermeable membranes are sandwiched in between these multilayered microfluidic microchannels. These membranes have different chemical, physical characteristics and MWCO values. Consequently, this design will produce much smaller dimension channels similar to human vasculature to achieve biomimetic properties like of human organs and tissues. By interchanging microfluidic-layers or the membranes various diverse modules are designed that act as building blocks for constructing various medical devices, various forms of dialysis devices including albumin and lipid dialysis, water purification, bioreactors, bio-artificial organ support systems. Connecting various modules in diverse combinations, permutations, in parallel and/or in series to ultimately design many unrelated medical devices such as dialysis, bioreactors and organ support devices.