Embodiments provide an apparatus and method for a membrane bioreactor process including a media in the water circulation module. Along with air supplied from bottom of the module the media components are kept in dynamic condition between the gaps to scrub the membrane surface area to it clean in-situ. Continued cleaning of the membrane surface results in benefits of reduced/no physical and chemical cleaning requirement, high flux, low TMP, and reduced frequency of chemical cleaning. The use of highly porous polymeric media having large internal surface area provides the advantage of retention of microbiological culture for a longer time without any disturbance causing an upset condition while increasing the biological loading and treatment capacity of the reactor.

An isolation device includes an isolation chip assembly, a vacuum system, a frequency converting module, and a controller. The isolation chip assembly includes an isolation chip having a first chamber and a second chamber, a first oscillator mounted on the first chamber, and a second oscillator mounted on the second chamber. The frequency converting module causes the vacuum system to generate negative pressure in the first and the second chambers alternately. The controller controls the first and the second oscillators to operate when the vacuum system stops generating the negative pressure in the first chamber and in the second chamber. The first and the second oscillators respectively generate a first and a second oscillation wave when operating, a frequency of the first oscillation wave is greater than a frequency of the oscillation wave, an amplitude of the first oscillation wave is less than an amplitude of the second oscillation wave.